Q8EAR9 (KDSA_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	SO_3827

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000187165

Sequences

	Sequence		Length	Mass (Da)
	Q8EAR9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 411A74BA2C9FC1E8	FASTA	282	30,951
	10 20 30 40 50 60 MSNKIIKLGS IEIANDKPFV LFGGMNVLES RDLAMSIAET YAEVTQKLGI PYVFKASFDK 70 80 90 100 110 120 ANRSSVNSYR GPGMEEGLKI FEEIKKTFNL PLITDVHETY QCAPVAEVVD IIQLPAFLAR 130 140 150 160 170 180 QTDLVVAMAK TGAIINVKKP QFLAPHEMRH IITKFNEAGN DEIILCERGS CFGYNNLVVD 190 200 210 220 230 240 MLGMDEMKQS GYPVIFDATH ALQRPGGRAD SAGGRRAQAT ELARSGMALG LAGLFIEAHP 250 260 270 280 DPDNAKCDGP CALPLHQLEN YLKQMKAIDD LVKSFEPIDT SK

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN56804.1.
RefSeq	NP_719360.1. NC_004347.1.
3D structure databases
ProteinModelPortal	Q8EAR9.
SMR	Q8EAR9. Positions 1-282.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1171472.
GenomeReviews	Gene locus SO_3827 in contig AE014299_GR.
KEGG	son:SO_3827.
NMPDR	fig|211586.1.peg.3476.
PATRIC	23527340. VBISheOne101494_3715.
TIGR	SO_3827.
Phylogenomic databases
HOGENOM	HBG575990.
OMA	HEPHQAA.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	SONE211586:SO_3827-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_SHEON

Accession

Primary (citable) accession number: Q8EAR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program