Reviewed, UniProtKB/Swiss-Prot Q8EAP2 (MAO1_SHEON)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	SO_3855

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity. HAMAP MF_01619
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160232
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EAP2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E6DA84E0EDFF8E0E	FASTA	562	62,162
	10 20 30 40 50 60 MDDNKRPLYL PFAGPAILEA PLINKGSAFS EEERIFFNLE GLVPYAIETI EEQASRAYDQ 70 80 90 100 110 120 FRSFNNDLDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGLACE RFSKNYRRNR 130 140 150 160 170 180 GLFISYPNKD RIDDILNNST RQKVKIIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG 190 200 210 220 230 240 GISPAYTLPI TLDVGTDNPQ LLEDPMYMGW RHPRIGGEEY AEFIEAFMQA VHVRWPDTLI 250 260 270 280 290 300 QFEDFAQKNA MPILERYKER YCCFNDDIQG TAAVTVGSLL AACKAAGTEL NKQRVAFLGA 310 320 330 340 350 360 GSAGCGIAEA IVAQMVSEGI SDEQARSQVC MVDRWGLLLD NMPNLLPFQQ KLAQKCADIS 370 380 390 400 410 420 HWNNFSDNIS LLDVVNNAKP TVLIGVSGAP GLFTEEIVRA MHSHCPRPII FPLSNPTSRV 430 440 450 460 470 480 EATPKDILHW TSGQALVATG SPFEPVVVDG ETYEIAQCNN SFIFPGIGLG VLASGARHVS 490 500 510 520 530 540 DAMLMASSRA LAECSPLAIN GSGPLLPKLE DIHSVSKHIA FAVGKVAIEQ GLSLPASDEL 550 560 LMQSIEDNFW KPEYRRYKRT SF

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN56831.1.
RefSeq	NP_719387.1.
3D structure databases
SMR	Q8EAP2. Positions 13-562.
ModBase	Search...
Genome annotation databases
GeneID	1171498.
GenomeReviews	Gene locus SO_3855 in contig AE014299_GR.
KEGG	son:SO_3855.
NMPDR	fig|211586.1.peg.3501.
TIGR	SO_3855.
Phylogenomic databases
HOGENOM	HBG289821.
OMA	NGYRISE.
Enzyme and pathway databases
BioCyc	SONE211586:SO_3855-MONOMER.
BRENDA	1.1.1.38. 257422.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_SHEON

Accession

Primary (citable) accession number: Q8EAP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)