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Reviewed, UniProtKB/Swiss-Prot Q8EAC7 (NRFA_SHEON)

Last modified June 16, 2009. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: SO_3980
OrganismShewanella oneidensis [Complete proteome] [HAMAP]
Taxonomic identifier70863 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

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Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity.

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity.

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 467440Cytochrome c-552 HAMAP MF_01182
PRO_0000268975

Sites

Metal binding871Iron (heme 3 axial ligand) By similarity
Metal binding1191Iron (heme 1 axial ligand) By similarity
Metal binding1571Iron (heme 2 axial ligand) By similarity
Metal binding1991Iron (heme 3 axial ligand) By similarity
Metal binding2011Calcium By similarity
Metal binding2021Calcium; via carbonyl oxygen By similarity
Metal binding2501Calcium; via carbonyl oxygen By similarity
Metal binding2521Calcium By similarity
Metal binding2641Iron (heme 5 axial ligand) By similarity
Metal binding2751Iron (heme 4 axial ligand) By similarity
Metal binding2901Iron (heme 2 axial ligand) By similarity
Metal binding3071Iron (heme 5 axial ligand) By similarity
Metal binding3821Iron (heme 4 axial ligand) By similarity
Binding site1151Heme 1 (covalent) By similarity
Binding site1181Heme 1 (covalent) By similarity
Binding site1531Heme 2 (covalent) By similarity
Binding site1561Heme 2 (covalent) By similarity
Binding site1951Heme 3 (covalent) By similarity
Binding site1981Heme 3 (covalent) By similarity
Binding site2021Substrate By similarity
Binding site2531Substrate By similarity
Binding site2711Heme 4 (covalent) By similarity
Binding site2741Heme 4 (covalent) By similarity
Binding site3031Heme 5 (covalent) By similarity
Binding site3061Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q8EAC7-1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 4BC976E90CDC48E0

FASTA46752,334
        10         20         30         40         50         60 
MMKKMTGKTF ALSALVAASF MAAGAMASDK TEPRNEVYKD KFKNQYNSWH DTAKSEELVD 

        70         80         90        100        110        120 
ALEQDPNMVI LWAGYAFAKD YKAPRGHMYA VTDVRNTLRT GAPKNAEDGP LPMACWSCKS 

       130        140        150        160        170        180 
PDVPRLIEEQ GEDGYFKGKW AKGGPEVTNT IGCSDCHEKG SPKLRISRPY VDRALDAIGT 

       190        200        210        220        230        240 
PFSKASKQDK ESMVCAQCHV EYYFEKKEDK KGFVKFPWDM GVTVDQMEVY YDGIEFSDWT 

       250        260        270        280        290        300 
HALSKTPMLK AQHPEYETWK MGIHGKNNVS CVDCHMPKVT SPEGKKFTDH KVGNPFDRFE 

       310        320        330        340        350        360 
ETCATCHSQT KEFLVGVTNE RKAKVKEMKL KAEEQLVKAH FEAAKAWELG ATEAEMKPIL 

       370        380        390        400        410        420 
TDIRHAQWRW DLAIASHGVA AHAPEEALRV LGTSVNKAAD ARVKLAQLLA KKGLTDPVAI 

       430        440        450        460 
PDISTKAKAQ AVLGMDMEKM NAEKEAFKKD MLPKWDAEAK KREATYK

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Cross-references

Sequence databases

AE014299 Genomic DNA. Translation: AAN56954.1.
RefSeqNP_719510.1.

3D structure databases

HSSPHSSP built from PDB template 1GU6 based on UniProtKB P32050.
ModBaseSearch...

Genome annotation databases

GeneID1171612.
GenomeReviewsGene locus SO_3980 in contig AE014299_GR.
KEGGson:SO_3980.
NMPDRfig|211586.1.peg.3613.
TIGRSO_3980.

Phylogenomic databases

HOGENOMQ8EAC7.
OMAQ8EAC7. CHDQSKE.

Enzyme and pathway databases

BioCycSONE211586:SO_3980-MON.
BRENDA1.7.2.2. 257422.

Family and domain databases

HAMAPMF_01182.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_SHEON
AccessionPrimary (citable) accession number: Q8EAC7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2003
Last modified: June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents