Q8E9D5 (ILVC_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	SO_4349

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151353
Sites
	Active site	132	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8E9D5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 72CA505178A935F4	FASTA	492	54,063
	10 20 30 40 50 60 MANYFNSLNL RQQLAQLGQC RFMDRSEFSD GCNYIKDWNI VILGCGAQGL NQGLNMRDSG 70 80 90 100 110 120 LNIAYALRPE AIAQKRASWQ KATDNGFKVG TFEELIPSAD LVLNLTPDKQ HSNVVSAVMP 130 140 150 160 170 180 LMKQGATLSY SHGFNIVEEG MQIRPDITVI MVAPKCPGTE VREEYKRGFG VPTLIAVHPE 190 200 210 220 230 240 NDPNGDGLDI AKAYASATGG DRAGVLQSSF IAEVKSDLMG EQTILCGMLQ TGAILGYEKM 250 260 270 280 290 300 VADGVEPGYA AKLIQQGWET VTEALKHGGI TNMMDRLSNP AKIKAFEIAE DLKDILQPLF 310 320 330 340 350 360 EKHMDDIISG EFSRTMMQDW ANDDANLLRW RAETGETGFE NAPVSSEHID EQTYFDKGIF 370 380 390 400 410 420 LVAMIKAGVE LAFDTMVSAG IVEESAYYES LHETPLIANT IARKRLYEMN VVISDTAEYG 430 440 450 460 470 480 CYLFNHAAVP LLRDYVNAMS PEYLGAGLKD SSNSVDNLQL IAINDAIRHT AVEYVGAELR 490 GYMTDMKSIV GA

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN57317.1.
RefSeq	NP_719873.1. NC_004347.1.
3D structure databases
ProteinModelPortal	Q8E9D5.
SMR	Q8E9D5. Positions 3-489.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1171952.
GenomeReviews	Gene locus SO_4349 in contig AE014299_GR.
KEGG	son:SO_4349.
NMPDR	fig|211586.1.peg.3948.
PATRIC	23528354. VBISheOne101494_4212.
TIGR	SO_4349.
Phylogenomic databases
HOGENOM	HBG297205.
OMA	EKHMDDI.
ProtClustDB	PRK05225.
Enzyme and pathway databases
BioCyc	SONE211586:SO_4349-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit. PTHR21371:SF2. PTHR21371:SF2. 1 hit.
Pfam	PF01450. IlvC. 2 hits. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_SHEON

Accession

Primary (citable) accession number: Q8E9D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program