Reviewed, UniProtKB/Swiss-Prot Q8E981 (KATG1_SHEON)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	SO_4405

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 728	706	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354920
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8E981-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 354F1B25226EF0E2	FASTA	728	80,981
	10 20 30 40 50 60 MDKTQRSQGK CPVMHGANSA VASNNMDWWP KALNLDILHQ HDQKTDPMDP NFSYRDAFNS 70 80 90 100 110 120 LDLAAVKQDL HSLMTDSQDW WPADWGHYGG LMIRMAWHSA GTYRIADGRG GAGTGNQRFA 130 140 150 160 170 180 PLNSWPDNAN LDKARRLLWP IKKKYGNKLS WADLIILAGN VAYESMGLKT FGFAGGRADI 190 200 210 220 230 240 WHPEKDIYWG SEKQWLAPSD NPNSRYSGAR DLENPLAAVM MGLIYVNPEG VDGKPDPLRT 250 260 270 280 290 300 AQDVRITFAR MAMDDEETVA LTAGGHTVGK CHGNGKAQDL GPEPEGEDVE AQGLGWLNKK 310 320 330 340 350 360 GRGVGCDAVT SGLEGAWTTH PTQWDNGYFY LLLNYDWELK KSPAGAWQWE PINIKEEDKV 370 380 390 400 410 420 ISVGDPAKKF NPIMTDADMA MKIDPEYRKI SERFYQDPAY FSEVFARAWF KLTHRDLGPK 430 440 450 460 470 480 SRYLGPEVPS EELIWQDPIP SVDYRLVASE IVELKAKLLA SGLSVSELVA TAWDSARTFR 490 500 510 520 530 540 GSDFRGGANG ARIRLAPQKD WSGNEPERLQ KVLKVLSALQ ASLSKKVSIA DLIVLGGAAA 550 560 570 580 590 600 IEKAAHAAGV NITVPFMPGR GDATKEMTDV ESFVVLEPLH DAYRNWLKQD YVVQPEEMML 610 620 630 640 650 660 DRSQLMGLTA HEMTVLIGGM RVLGTNHGGT RHGVFTDKVG VLSNDFFVNL TDMAYSWKPV 670 680 690 700 710 720 GTNLYEVVER KTGTVKWTAT RVDLVFGSNS VLRVYAEVYA QDDAKEKFVH DFVAAWTKVM NADRFDLA

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014299 Genomic DNA. Translation: AAN57372.1.
RefSeq	NP_719928.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Protein family/group databases
PeroxiBase	2660. SonCP02.
Genome annotation databases
GeneID	1172005.
GenomeReviews	Gene locus SO_4405 in contig AE014299_GR.
KEGG	son:SO_4405.
NMPDR	fig|211586.1.peg.4000.
TIGR	SO_4405.
Phylogenomic databases
HOGENOM	Q8E981.
OMA	Q8E981. WPNALNL.
Enzyme and pathway databases
BioCyc	SONE211586:SO_4405-MON.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_SHEON

Accession

Primary (citable) accession number: Q8E981

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	March 1, 2003
Last modified:	May 5, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)