Q8E7X8 (KPRS1_STRA3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 1
Short name=RPPK 1
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 1
Short name=P-Rib-PP synthase 1
Short name=PRPP synthase 1

Gene names

Name:	prs1
Ordered Locus Names:	gbs0017

Organism

Streptococcus agalactiae serotype III [Complete proteome] [HAMAP]

Taxonomic identifier

216495 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Ribose-phosphate pyrophosphokinase 1 HAMAP MF_00583_B		PRO_0000141196
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	130	1	Magnesium Potential
	Metal binding	132	1	Magnesium Potential
	Metal binding	141	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8E7X8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2B89EDCC18092505	FASTA	322	35,284
	10 20 30 40 50 60 MSYSNLKLFA LSSNKELAKK VSQTIGIPLG QSTVRQFSDG EIQVNIEESI RGHHVFILQS 70 80 90 100 110 120 TSSPVNDNLM EILIMVDALK RASAESVSVV MPYYGYARQD RKARSREPIT SKLVANMLEV 130 140 150 160 170 180 AGVDRLLTVD LHAAQIQGFF DIPVDHLMGA PLIADYFDRQ GLVGDDVVVV SPDHGGVTRA 190 200 210 220 230 240 RKLAQCLKTP IAIIDKRRSV TKMNTSEVMN IIGNIKGKKC ILIDDMIDTA GTICHAADAL 250 260 270 280 290 300 AEAGATAVYA SCTHPVLSGP ALDNIQNSAI EKLIVLDTIY LPEERLIDKI EQISIAELIG 310 320 EAIIRIHEKR PLSPLFEMNK LK

References

[1]	"Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease." Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F. Mol. Microbiol. 45:1499-1513(2002) [PubMed: 12354221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NEM316 / Serotype III.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL766843 Genomic DNA. Translation: CAD45662.1.
RefSeq	NP_734487.1. NC_004368.1.
3D structure databases
ProteinModelPortal	Q8E7X8.
SMR	Q8E7X8. Positions 5-316.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000003515; EBSTRP00000003433; EBSTRG00000003515.
GeneID	1031120.
GenomeReviews	Gene locus gbs0017 in contig AL732656_GR.
KEGG	san:gbs0017.
PATRIC	19635737. VBIStrAga3577_0017.
Organism-specific databases
GenoList	gbs0017.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027352.
HOGENOM	HBG519284.
OMA	CATHAVF.
ProtClustDB	PRK02269.
Enzyme and pathway databases
BioCyc	SAGA211110:GBS0017-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_STRA3

Accession

Primary (citable) accession number: Q8E7X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program