Reviewed, UniProtKB/Swiss-Prot Q8E6A9 (LDHD_STRA3)

Names and origin

Protein names

Recommended name:
    D-lactate dehydrogenase
      Short name=D-LDH
    EC=1.1.1.28
Alternative name(s):
    D-specific D-2-hydroxyacid dehydrogenase

Gene names

Name:	ldhD
Ordered Locus Names:	gbs0668

Organism

Streptococcus agalactiae serotype III [Complete proteome] [HAMAP]

Taxonomic identifier

216495 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	D-lactate dehydrogenase		PRO_0000075968
Regions
	Nucleotide binding	155 – 156	2	NAD By similarity
	Nucleotide binding	233 – 235	3	NAD By similarity
	Nucleotide binding	296 – 299	4	NAD By similarity
Sites
	Active site	235	1	By similarity
	Active site	264	1	By similarity
	Active site	296	1	Proton donor By similarity
	Binding site	259	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8E6A9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1E639C912B4A46B5	FASTA	330	36,501
	10 20 30 40 50 60 MKLKVFNVRE EEATLAQDWA NRNHVELSMS EGPLTLETVN EVEGFDGIAN AQIGPLDDAI 70 80 90 100 110 120 YPLLKEMGIK QIAQRSAGVD MYNLELAKQH GIIISNVPSY SPESIAEFTV TIALNLIRKV 130 140 150 160 170 180 ELIRANVREQ NFSWTLPIRG RVLGNMTVAI IGTGRIGLAT AKIFKGFGCR VIGYDIYHNP 190 200 210 220 230 240 MADGILEYVN SVEEAVEKAD LVSLHMPPTA ENTHLFNLDM FKQFKKGAIL MNMARGALVE 250 260 270 280 290 300 TKDLLEALDQ GLLEGAGIDT YEFEGPYIPK NCQGQDISDK DFLRLINHPK VIYTPHAAYY 310 320 330 TDEAVKNLVE GALNACVEVV ETGTTTTRVN

References

[1]

"Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease." Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F. Mol. Microbiol. 45:1499-1513(2002) [PubMed: 12354221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NEM316 / Serotype III.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL766846 Genomic DNA. Translation: CAD46312.1.
RefSeq	NP_735118.1.
3D structure databases
SMR	Q8E6A9. Positions 1-330.
ModBase	Search...
Genome annotation databases
GeneID	1031708.
KEGG	san:gbs0668.
Organism-specific databases
SagaList	gbs0668.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG731446.
OMA	KWGERAS.
Enzyme and pathway databases
BioCyc	SAGA211110:GBS0668-MONOMER.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_STRA3

Accession

Primary (citable) accession number: Q8E6A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)