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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Streptococcus agalactiae serotype III (strain NEM316)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi459 – 4591MagnesiumUniRule annotation
Metal bindingi465 – 4651Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi468 – 4681MagnesiumUniRule annotation
Metal bindingi469 – 4691MagnesiumUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
Short name:
PheRSUniRule annotation
Gene namesi
Name:pheTUniRule annotation
Ordered Locus Names:gbs0888
OrganismiStreptococcus agalactiae serotype III (strain NEM316)
Taxonomic identifieri211110 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000823 Componenti: Chromosome

Organism-specific databases

GenoListigbs0888.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 801801Phenylalanine--tRNA ligase beta subunitPRO_0000126957Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi211110.gbs0888.

Structurei

3D structure databases

ProteinModelPortaliQ8E5U1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 153115tRNA-bindingUniRule annotationAdd
BLAST
Domaini406 – 48176B5UniRule annotationAdd
BLAST
Domaini708 – 80194FDX-ACBUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
Contains 1 B5 domain.UniRule annotation
Contains 1 FDX-ACB domain.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292087.
KOiK01890.
OMAiRQYGLHT.
OrthoDBiEOG6CCH1J.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8E5U1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVSYKWLKE LVDVDVTTAE LAEKMSTTGI EVEGVETPAE GLSKLVVGHI
60 70 80 90 100
VSCEDVPDTH LHLCQVDTGD DELRQVVCGA PNVKTGINVI VAVPGARIAD
110 120 130 140 150
NYKIKKGKIR GMESLGMICS LQELGLSESI IPKEFSDGIQ ILPEGAIPGD
160 170 180 190 200
SIFSYLDLDD EIIELSITPN RADALSMRGV AHEVAAIYGK KVHFEEKNLI
210 220 230 240 250
EEAERAADKI SVVIESDKVL SYSARIVKNV TVAPSPQWLQ NKLMNAGIRP
260 270 280 290 300
INNVVDVTNY VLLTYGQPMH AFDFDKFDGT TIVARNAENG EKLITLDGEE
310 320 330 340 350
RDLIADDLVI AVNDQPVALA GVMGGQSTEI GSSSKTVVLE AAVFNGTSIR
360 370 380 390 400
KTSGRLNLRS ESSSRFEKGI NYDTVSEAMD FAAAMLQELA GGQVLSGQVT
410 420 430 440 450
EGVLPTEPVE VSTTLGYVNT RLGTELTYTD IEEVFEKLGF AISGSEVKFT
460 470 480 490 500
VLVPRRRWDI AIQADLVEEI ARIYGYEKLP TTLPEAGATA GELTSMQRLR
510 520 530 540 550
RRVRTVAEGA GLSEIITYAL TTPEKAVQFS TQATNITELM WPMTVDRSAL
560 570 580 590 600
RQNVVSGMLD TIAYNVARKN SNLAVYEIGK VFEQTGNPKE DLPTEVETFT
610 620 630 640 650
FALTGLVEEK DFQTKAKPVD FFYAKGIVEA LFIKLKLDVT FVAQKGLASM
660 670 680 690 700
HPGRTATILL DGKEIGFVGQ VHPQTAKQYD IPETYVAEIN LSTIESQMNQ
710 720 730 740 750
ALIFEDITKY PSVSRDIALL LAESVSHHDI VSAIETSGVK RLTAIKLFDV
760 770 780 790 800
YAGNNIAEGY KSMAYSLTFQ NPNDNLTDEE VAKYMEKITK SLVEKVNAEI

R
Length:801
Mass (Da):87,622
Last modified:March 1, 2003 - v1
Checksum:iD0E776E9D0084CEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL766847 Genomic DNA. Translation: CAD46532.1.
RefSeqiNP_735338.1. NC_004368.1.

Genome annotation databases

KEGGisan:gbs0888.
PATRICi19637640. VBIStrAga3577_0912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL766847 Genomic DNA. Translation: CAD46532.1.
RefSeqiNP_735338.1. NC_004368.1.

3D structure databases

ProteinModelPortaliQ8E5U1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi211110.gbs0888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGisan:gbs0888.
PATRICi19637640. VBIStrAga3577_0912.

Organism-specific databases

GenoListigbs0888.

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292087.
KOiK01890.
OMAiRQYGLHT.
OrthoDBiEOG6CCH1J.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease."
    Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.
    Mol. Microbiol. 45:1499-1513(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NEM316.

Entry informationi

Entry nameiSYFB_STRA3
AccessioniPrimary (citable) accession number: Q8E5U1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2003
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.