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Q8E5U1

- SYFB_STRA3

UniProt

Q8E5U1 - SYFB_STRA3

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Streptococcus agalactiae serotype III (strain NEM316)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

    Cofactori

    Binds 2 magnesium ions per tetramer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi459 – 4591MagnesiumUniRule annotation
    Metal bindingi465 – 4651Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi468 – 4681MagnesiumUniRule annotation
    Metal bindingi469 – 4691MagnesiumUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
    Short name:
    PheRSUniRule annotation
    Gene namesi
    Name:pheTUniRule annotation
    Ordered Locus Names:gbs0888
    OrganismiStreptococcus agalactiae serotype III (strain NEM316)
    Taxonomic identifieri211110 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000823: Chromosome

    Organism-specific databases

    GenoListigbs0888.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 801801Phenylalanine--tRNA ligase beta subunitPRO_0000126957Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi211110.gbs0888.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8E5U1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 153115tRNA-bindingUniRule annotationAdd
    BLAST
    Domaini406 – 48176B5UniRule annotationAdd
    BLAST
    Domaini708 – 80194FDX-ACBUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
    Contains 1 B5 domain.UniRule annotation
    Contains 1 FDX-ACB domain.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0072.
    HOGENOMiHOG000292087.
    KOiK01890.
    OMAiNYVMIEL.
    OrthoDBiEOG6CCH1J.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8E5U1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLVSYKWLKE LVDVDVTTAE LAEKMSTTGI EVEGVETPAE GLSKLVVGHI    50
    VSCEDVPDTH LHLCQVDTGD DELRQVVCGA PNVKTGINVI VAVPGARIAD 100
    NYKIKKGKIR GMESLGMICS LQELGLSESI IPKEFSDGIQ ILPEGAIPGD 150
    SIFSYLDLDD EIIELSITPN RADALSMRGV AHEVAAIYGK KVHFEEKNLI 200
    EEAERAADKI SVVIESDKVL SYSARIVKNV TVAPSPQWLQ NKLMNAGIRP 250
    INNVVDVTNY VLLTYGQPMH AFDFDKFDGT TIVARNAENG EKLITLDGEE 300
    RDLIADDLVI AVNDQPVALA GVMGGQSTEI GSSSKTVVLE AAVFNGTSIR 350
    KTSGRLNLRS ESSSRFEKGI NYDTVSEAMD FAAAMLQELA GGQVLSGQVT 400
    EGVLPTEPVE VSTTLGYVNT RLGTELTYTD IEEVFEKLGF AISGSEVKFT 450
    VLVPRRRWDI AIQADLVEEI ARIYGYEKLP TTLPEAGATA GELTSMQRLR 500
    RRVRTVAEGA GLSEIITYAL TTPEKAVQFS TQATNITELM WPMTVDRSAL 550
    RQNVVSGMLD TIAYNVARKN SNLAVYEIGK VFEQTGNPKE DLPTEVETFT 600
    FALTGLVEEK DFQTKAKPVD FFYAKGIVEA LFIKLKLDVT FVAQKGLASM 650
    HPGRTATILL DGKEIGFVGQ VHPQTAKQYD IPETYVAEIN LSTIESQMNQ 700
    ALIFEDITKY PSVSRDIALL LAESVSHHDI VSAIETSGVK RLTAIKLFDV 750
    YAGNNIAEGY KSMAYSLTFQ NPNDNLTDEE VAKYMEKITK SLVEKVNAEI 800
    R 801
    Length:801
    Mass (Da):87,622
    Last modified:March 1, 2003 - v1
    Checksum:iD0E776E9D0084CEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL766847 Genomic DNA. Translation: CAD46532.1.
    RefSeqiNP_735338.1. NC_004368.1.

    Genome annotation databases

    EnsemblBacteriaiCAD46532; CAD46532; CAD46532.
    GeneIDi1029872.
    KEGGisan:gbs0888.
    PATRICi19637640. VBIStrAga3577_0912.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL766847 Genomic DNA. Translation: CAD46532.1 .
    RefSeqi NP_735338.1. NC_004368.1.

    3D structure databases

    ProteinModelPortali Q8E5U1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 211110.gbs0888.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD46532 ; CAD46532 ; CAD46532 .
    GeneIDi 1029872.
    KEGGi san:gbs0888.
    PATRICi 19637640. VBIStrAga3577_0912.

    Organism-specific databases

    GenoListi gbs0888.

    Phylogenomic databases

    eggNOGi COG0072.
    HOGENOMi HOG000292087.
    KOi K01890.
    OMAi NYVMIEL.
    OrthoDBi EOG6CCH1J.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease."
      Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.
      Mol. Microbiol. 45:1499-1513(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NEM316.

    Entry informationi

    Entry nameiSYFB_STRA3
    AccessioniPrimary (citable) accession number: Q8E5U1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3