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Q8E4L6 (TYSY_STRA3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:gbs1385
OrganismStreptococcus agalactiae serotype III (strain NEM316) [Complete proteome] [HAMAP]
Taxonomic identifier211110 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000141027

Sites

Active site1541 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8E4L6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 865F880A56F01642

FASTA27932,527
        10         20         30         40         50         60 
MTKADLLFKD NITKIMSEGV FSEQARPRYK NGEMANSKYI TGAFAEYDLS KGEFPITTLR 

        70         80         90        100        110        120 
PIPIKSAIKE IFWIYQDQTN DLVVLNDKYG VTYWNDWEVG HTGTIGQRYG AVVKKHNIIS 

       130        140        150        160        170        180 
KLLKQLEDNP WNRRNVISLW DYEAFEETEG LLPCAFQTMF DVRRVNGELY LDATLTQRSN 

       190        200        210        220        230        240 
DMLVAHHINA MQYVALQMMI AKHFGWRVGK FFYFINNLHI YDNQFEQAQE LLKRQPSECN 

       250        260        270 
PKLVLNVPDG TDFFDIKPDD FALVDYDPIK PQLRFDLAI 

« Hide

References

[1]"Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease."
Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.
Mol. Microbiol. 45:1499-1513(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NEM316.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL766850 Genomic DNA. Translation: CAD47044.1.
RefSeqNP_735822.1. NC_004368.1.

3D structure databases

ProteinModelPortalQ8E4L6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211110.gbs1385.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD47044; CAD47044; CAD47044.
GeneID1030432.
KEGGsan:gbs1385.
PATRIC19638655. VBIStrAga3577_1416.

Organism-specific databases

GenoListgbs1385.
CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257898.
KOK00560.
OMAANPWNRR.
OrthoDBEOG6K6V53.
ProtClustDBPRK01827.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_STRA3
AccessionPrimary (citable) accession number: Q8E4L6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways