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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Streptococcus agalactiae serotype III (strain NEM316)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
  2. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi696Magnesium or manganese 1UniRule annotation1
Metal bindingi717Magnesium or manganese 1UniRule annotation1
Metal bindingi717Magnesium or manganese 2UniRule annotation1
Metal bindingi719Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi516 – 574ATPUniRule annotationAdd BLAST59

GO - Molecular functioni

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209
UPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:gbs1862
OrganismiStreptococcus agalactiae serotype III (strain NEM316)
Taxonomic identifieri211110 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000823 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925581 – 750Glutathione biosynthesis bifunctional protein GshABAdd BLAST750

Proteomic databases

PRIDEiQ8E399

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8E399
SMRiQ8E399
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini489 – 747ATP-graspUniRule annotationAdd BLAST259

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 333Glutamate--cysteine ligaseAdd BLAST333

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9M Bacteria
COG1181 LUCA
COG2918 LUCA
HOGENOMiHOG000156471
KOiK01919
OMAiEANFNPM

Family and domain databases

HAMAPiMF_00782 Glut_biosynth, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR007370 Glu_cys_ligase
IPR006335 Glut_biosynth
IPR006334 Glut_cys_ligase
IPR020561 PRibGlycinamid_synth_ATP-grasp
PANTHERiPTHR38761 PTHR38761, 2 hits
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF04262 Glu_cys_ligase, 1 hit
SUPFAMiSSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR01435 glu_cys_lig_rel, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8E399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIDRLLQRS HSHLPILQAT FGLERESLRI HQPTQRVAQT PHPKTLGSRN
60 70 80 90 100
YHPYIQTDYS EPQLELITPI AKDSQEAIRF LKAISDVAGR SINHDEYLWP
110 120 130 140 150
LSMPPKVREE DIQIAQLEDA FEYDYRKYLE KTYGKLIQSI SGIHYNLGLG
160 170 180 190 200
QELLTSLFEL SQADNAIDFQ NQLYMKLSQN FLRYRWLLTY LYGASPVAEE
210 220 230 240 250
DFLDQKLNNP VRSLRNSHLG YVNHKDIRIS YTSLKDYVND LENAVKSGQL
260 270 280 290 300
IAEKEFYSPV RLRGSKACRN YLEKGITYLE FRTFDLNPFS PIGITQETVD
310 320 330 340 350
TVHLFLLALL WIDSSSHIDQ DIKEANRLND LIALSHPLEK LPNQAPVSDL
360 370 380 390 400
VDAMQSVIQH FNLSPYYQDL LESVKRQIQS PELTVAGQLL EMIEGLSLET
410 420 430 440 450
FGQRQGQIYH DYAWEAPYAL KGYETMELST QLLLFDVIQK GVNFEVLDEQ
460 470 480 490 500
DQFLKLWHNS HIEYVKNGNM TSKDNYIVPL AMANKVVTKK ILDEKHFPTP
510 520 530 540 550
FGDEFTDRKE ALNYFSQIQD KPIVVKPKST NFGLGISIFK TSANLASYEK
560 570 580 590 600
AIDIAFTEDS AILVEEYIEG TEYRFFVLEG DCIAVLLRVA ANVVGDGIHT
610 620 630 640 650
ISQLVKLKNQ NPLRGYDHRS PLEVIELGEV EQLMLEQQGY TVNSIPPEGT
660 670 680 690 700
KIELRRNSNI STGGDSIDVT NTMDPTYKQL AAEMAEAMGA WVCGVDLIIP
710 720 730 740 750
NATQAYSKDK KNATCIELNF NPLMYMHTYC QEGPGQSITP RILAKLFPEL
Length:750
Mass (Da):85,603
Last modified:March 1, 2003 - v1
Checksum:i97829C2C5B44174A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL766854 Genomic DNA Translation: CAD47521.1
RefSeqiWP_000582678.1, NC_004368.1

Genome annotation databases

EnsemblBacteriaiCAD47521; CAD47521; CAD47521
KEGGisan:gbs1862

Entry informationi

Entry nameiGSHAB_STRA3
AccessioniPrimary (citable) accession number: Q8E399
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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