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Q8E362 (SYP_STRA3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proline--tRNA ligase

EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS
Gene names
Name:proS
Ordered Locus Names:gbs1900
OrganismStreptococcus agalactiae serotype III (strain NEM316) [Complete proteome] [HAMAP]
Taxonomic identifier211110 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length617 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP-Rule MF_01569

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01569

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01569

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01569.

Domain

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP-Rule MF_01569

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

proline-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 617617Proline--tRNA ligase HAMAP-Rule MF_01569
PRO_0000248774

Sequences

Sequence LengthMass (Da)Tools
Q8E362 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 01D99B3116B32D57

FASTA61769,024
        10         20         30         40         50         60 
MKQSKMLIPT LREMPSDAQV ISHALMVRAG YVRQVSAGIY AYLPLANRTI EKFKTIMRQE 

        70         80         90        100        110        120 
FEKIGAVEML APALLTADLW RESGRYETYG EDLYKLKNRD QSDFILGPTH EETFTTLVRD 

       130        140        150        160        170        180 
AVKSYKQLPL NLYQIQSKYR DEKRPRNGLL RTREFIMKDG YSFHKDYEDL DVTYEDYRKA 

       190        200        210        220        230        240 
YEAIFTRAGL DFKGIIGDGG AMGGKDSQEF MAVTPNRTDL NRWLVLDKTI PSIDDIPEDV 

       250        260        270        280        290        300 
LEEIKVELSA WLVSGEDTIA YSTESSYAAN LEMATNEYKP STKAATFEEV TRVETPNCKS 

       310        320        330        340        350        360 
IDEVAGFLSI DENQTIKTLL FIADEQPVVA LLVGNDQVND VKLKNYLAAD FLEPASEEQA 

       370        380        390        400        410        420 
KEIFGAGFGS LGPVNLPDSV KIIADRKVQD LANAVSGANQ DGYHFTGVNP ERDFTAEYVD 

       430        440        450        460        470        480 
IREVKEGEIS PDGKGTLKFA RGIEIGHIFK LGTRYSDSMG ANILDENGRS NPIVMGCYGI 

       490        500        510        520        530        540 
GVSRILSAVI EQHARLFVNK TPKGAYRFAW GINFPEELAP FDVHLITVNV KDQESQDLTE 

       550        560        570        580        590        600 
KIEADLMLKG YEVLTDDRNE RVGSKFSDSD LIGLPIRVTV GKKASEGIVE VKIKASGDTI 

       610 
EVHADNLIET LEILTKK 

« Hide

References

[1]"Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease."
Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.
Mol. Microbiol. 45:1499-1513(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NEM316.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL766854 Genomic DNA. Translation: CAD47559.1.
RefSeqNP_736334.1. NC_004368.1.

3D structure databases

ProteinModelPortalQ8E362.
SMRQ8E362. Positions 1-614.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211110.gbs1900.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD47559; CAD47559; CAD47559.
GeneID1031002.
KEGGsan:gbs1900.
PATRIC19639723. VBIStrAga3577_1947.

Organism-specific databases

GenoListgbs1900.
CMRSearch...

Phylogenomic databases

eggNOGCOG0442.
HOGENOMHOG000076893.
KOK01881.
OMAIQPAELW.
OrthoDBEOG6TTVMR.
ProtClustDBPRK09194.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPMF_01569. Pro_tRNA_synth_type1.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsTIGR00409. proS_fam_II. 2 hits.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP_STRA3
AccessionPrimary (citable) accession number: Q8E362
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries