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Q8E362

- SYP_STRA3

UniProt

Q8E362 - SYP_STRA3

Protein

Proline--tRNA ligase

Gene

proS

Organism
Streptococcus agalactiae serotype III (strain NEM316)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetaseUniRule annotation
    Short name:
    ProRSUniRule annotation
    Gene namesi
    Name:proSUniRule annotation
    Ordered Locus Names:gbs1900
    OrganismiStreptococcus agalactiae serotype III (strain NEM316)
    Taxonomic identifieri211110 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000823: Chromosome

    Organism-specific databases

    GenoListigbs1900.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 617617Proline--tRNA ligasePRO_0000248774Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi211110.gbs1900.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8E362.
    SMRiQ8E362. Positions 1-614.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0442.
    HOGENOMiHOG000076893.
    KOiK01881.
    OMAiMHQAYCN.
    OrthoDBiEOG6TTVMR.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 2 hits.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8E362-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQSKMLIPT LREMPSDAQV ISHALMVRAG YVRQVSAGIY AYLPLANRTI    50
    EKFKTIMRQE FEKIGAVEML APALLTADLW RESGRYETYG EDLYKLKNRD 100
    QSDFILGPTH EETFTTLVRD AVKSYKQLPL NLYQIQSKYR DEKRPRNGLL 150
    RTREFIMKDG YSFHKDYEDL DVTYEDYRKA YEAIFTRAGL DFKGIIGDGG 200
    AMGGKDSQEF MAVTPNRTDL NRWLVLDKTI PSIDDIPEDV LEEIKVELSA 250
    WLVSGEDTIA YSTESSYAAN LEMATNEYKP STKAATFEEV TRVETPNCKS 300
    IDEVAGFLSI DENQTIKTLL FIADEQPVVA LLVGNDQVND VKLKNYLAAD 350
    FLEPASEEQA KEIFGAGFGS LGPVNLPDSV KIIADRKVQD LANAVSGANQ 400
    DGYHFTGVNP ERDFTAEYVD IREVKEGEIS PDGKGTLKFA RGIEIGHIFK 450
    LGTRYSDSMG ANILDENGRS NPIVMGCYGI GVSRILSAVI EQHARLFVNK 500
    TPKGAYRFAW GINFPEELAP FDVHLITVNV KDQESQDLTE KIEADLMLKG 550
    YEVLTDDRNE RVGSKFSDSD LIGLPIRVTV GKKASEGIVE VKIKASGDTI 600
    EVHADNLIET LEILTKK 617
    Length:617
    Mass (Da):69,024
    Last modified:March 1, 2003 - v1
    Checksum:i01D99B3116B32D57
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL766854 Genomic DNA. Translation: CAD47559.1.
    RefSeqiNP_736334.1. NC_004368.1.

    Genome annotation databases

    EnsemblBacteriaiCAD47559; CAD47559; CAD47559.
    GeneIDi1031002.
    KEGGisan:gbs1900.
    PATRICi19639723. VBIStrAga3577_1947.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL766854 Genomic DNA. Translation: CAD47559.1 .
    RefSeqi NP_736334.1. NC_004368.1.

    3D structure databases

    ProteinModelPortali Q8E362.
    SMRi Q8E362. Positions 1-614.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 211110.gbs1900.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD47559 ; CAD47559 ; CAD47559 .
    GeneIDi 1031002.
    KEGGi san:gbs1900.
    PATRICi 19639723. VBIStrAga3577_1947.

    Organism-specific databases

    GenoListi gbs1900.

    Phylogenomic databases

    eggNOGi COG0442.
    HOGENOMi HOG000076893.
    KOi K01881.
    OMAi MHQAYCN.
    OrthoDBi EOG6TTVMR.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 2 hits.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease."
      Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.
      Mol. Microbiol. 45:1499-1513(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NEM316.

    Entry informationi

    Entry nameiSYP_STRA3
    AccessioniPrimary (citable) accession number: Q8E362
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3