Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 31ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAGA208435:G1FZP-14-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:SAG0014
OrganismiStreptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Taxonomic identifieri208435 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000821 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001817741 – 424tRNA(Ile)-lysidine synthaseAdd BLAST424

Structurei

3D structure databases

ProteinModelPortaliQ8E2H4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000235102
KOiK04075
OMAiTFRYEFF

Family and domain databases

CDDicd01992 PP-ATPase, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_01161 tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR012796 Lysidine-tRNA-synth_C
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012094 tRNA_Ile_lys_synt
IPR012795 tRNA_Ile_lys_synt_N
PANTHERiPTHR43033:SF1 PTHR43033:SF1, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
SMARTiView protein in SMART
SM00977 TilS_C, 1 hit
TIGRFAMsiTIGR02433 lysidine_TilS_C, 1 hit
TIGR02432 lysidine_TilS_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q8E2H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNTILKDTL SKGLFTAHQK VLIAVSGGID SINLLQFLYQ YQKELSISIG
60 70 80 90 100
IAHINHGQRK ESEKEEEYIR QWGQIHDVPV FISYFQGIFS EDRARNHRYN
110 120 130 140 150
FFSKVMREEG YTALVTAHHA DDQAETVFMR ILRGSRLRYL SGIKQVSAFA
160 170 180 190 200
NGQLIRPFLP YKKELLPNIF HFEDASNASS DYLRNRIRNV YFPALERENN
210 220 230 240 250
QLKDSLITLS EETECLFTAL TDLTRSIEVT NCYDFLRQTH SVQEFLLQDY
260 270 280 290 300
ISKFPDLQVS KEQFRVILKL IRTKANIDYT IKSGYFLHKD YESFHITKIH
310 320 330 340 350
PKTDSFKVEK RLELHNIQIF SQYLFSYGKF ISQADITIPI YDTSPIILRR
360 370 380 390 400
RKEGDRIFLG NHTKKIRRLF IDEKITLKER EEAVIGEQNK ELIFVIVAGR
410 420
TYLRKPSEHD IMKGKLYIEN LEKR
Length:424
Mass (Da):49,933
Last modified:March 1, 2003 - v1
Checksum:i3B42CFBE1EED7C62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009948 Genomic DNA Translation: AAM98922.1
RefSeqiNP_687050.1, NC_004116.1
WP_000282856.1, NC_004116.1

Genome annotation databases

EnsemblBacteriaiAAM98922; AAM98922; SAG0014
GeneIDi1012732
KEGGisag:SAG0014
PATRICifig|208435.3.peg.13

Similar proteinsi

Entry informationi

Entry nameiTILS_STRA5
AccessioniPrimary (citable) accession number: Q8E2H4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health