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Q8E177

- SYI_STRA5

UniProt

Q8E177 - SYI_STRA5

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Protein
Isoleucine--tRNA ligase
Gene
ileS, SAG0485
Organism
Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylate By similarity
Binding sitei598 – 5981ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAGA208435:GHVY-575-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SAG0485
OrganismiStreptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Taxonomic identifieri208435 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000821: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098477Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi208435.SAG0485.

Structurei

3D structure databases

ProteinModelPortaliQ8E177.
SMRiQ8E177. Positions 1-918.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8E177-1 [UniParc]FASTAAdd to Basket

« Hide

MKLKETLNLG QTAFPMRAGL PNKEPQWQEA WDQADIYKKR QALNEGKPAF    50
HLHDGPPYAN GNIHVGHALN KISKDIIVRS KSMSGFRAPY VPGWDTHGLP 100
IEQVLAKKGV KRKEMDLAEY LEMCRDYALS QVDKQRDDFK RLGVSADWEN 150
PYITLTPDYE ADQVRVFGAM ADKGYIYRGA KPVYWSWSSE SALAEAEIEY 200
HDIDSTSLYY ANKVKDGKGI LDTDTYIVVW TTTPFTVTAS RGLTVGPDME 250
YVVVVPVGSE RKYLLAEVLV DSLAAKFGWE NFEIVTHHTG KELNHIVTEH 300
PWDTEVEELV ILGDHVTTDS GTGIVHTAPG FGEDDYNVGI ANGLDVVVTV 350
DSRGLMMENA GPDFEGQFYD KVTPLVKEKL GDLLLASEVI NHSYPFDWRT 400
KKPIIWRAVP QWFASVSKFR QEILDEIEKT NFQPEWGKKR LYNMIRDRGD 450
WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHVADLFAEY GSIVWWQRDA 500
KDLLPAGYTH PGSPNGLFEK ETDIMDVWFD SGSSWNGVMN ARENLSYPAD 550
LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AVLSQGFVLD GKGEKMSKSL 600
GNTILPSDVE KQFGAEILRL WVTSVDSSND VRISMDILKQ TSETYRKIRN 650
TLRFLIANTS DFNPKQDAVA YENLGAVDRY MTIKFNQVVD TINKAYAAYD 700
FMAIYKAVVN FVTVDLSAFY LDFAKDVVYI EAANSPERRR MQTVFYDILV 750
KLTKLLTPIL PHTAEEIWSY LEHEEEEFVQ LAEMPVAQTF SGQEEILEEW 800
SAFMTLRTQA QKALEEARNA KVIGKSLEAH LTIYASQEVK TLLTALNSDI 850
ALLMIVSQLT IADEADKPAD SVSFEGVAFT VEHAEGEVCE RSRRIDPTTK 900
MRSYGVAVCD ASAAIIEQYY PEAVAQGFEA 930
Length:930
Mass (Da):104,897
Last modified:March 1, 2003 - v1
Checksum:iC77646FA00A10CDE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009948 Genomic DNA. Translation: AAM99387.1.
RefSeqiNP_687515.1. NC_004116.1.

Genome annotation databases

EnsemblBacteriaiAAM99387; AAM99387; SAG0485.
GeneIDi1013288.
KEGGisag:SAG0485.
PATRICi19627782. VBIStrAga72745_0483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009948 Genomic DNA. Translation: AAM99387.1 .
RefSeqi NP_687515.1. NC_004116.1.

3D structure databases

ProteinModelPortali Q8E177.
SMRi Q8E177. Positions 1-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208435.SAG0485.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM99387 ; AAM99387 ; SAG0485 .
GeneIDi 1013288.
KEGGi sag:SAG0485.
PATRICi 19627782. VBIStrAga72745_0483.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SAGA208435:GHVY-575-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-611 / 2603 V/R.

Entry informationi

Entry nameiSYI_STRA5
AccessioniPrimary (citable) accession number: Q8E177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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