ID   Q8E0S5_STRA5            Unreviewed;       890 AA.
AC   Q8E0S5;
DT   01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2003, sequence version 1.
DT   27-MAR-2024, entry version 102.
DE   SubName: Full=Cell wall surface anchor family protein, putative {ECO:0000313|EMBL:AAM99541.1};
GN   OrderedLocusNames=SAG0649 {ECO:0000313|EMBL:AAM99541.1};
OS   Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R).
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=208435 {ECO:0000313|EMBL:AAM99541.1, ECO:0000313|Proteomes:UP000000821};
RN   [1] {ECO:0000313|EMBL:AAM99541.1, ECO:0000313|Proteomes:UP000000821}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-611 / 2603 V/R {ECO:0000313|Proteomes:UP000000821};
RX   PubMed=12200547; DOI=10.1073/pnas.182380799;
RA   Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S.,
RA   Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D.,
RA   Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   DeBoy R.T., Durkin S., Kolonay J.F., Umayam L.A., Madupu R., Lewis M.R.,
RA   Radune D., Fedorova N.B., Scanlan D., Khouri H., Mulligan S., Carty H.A.,
RA   Cline R.T., Gill J., Scarselli M., Mora M., Iacobini E.T., Brettoni C.,
RA   Galli G., Mariani M., Vegni F., Maione D., Rinaudo D., Rappuoli R.,
RA   Telford J.L., Kasper D.L., Grandi G., Fraser C.M.;
RT   "Complete genome sequence and comparative genomic analysis of an emerging
RT   human pathogen, serotype V Streptococcus agalactiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:12391-12396(2002).
RN   [2] {ECO:0007829|PDB:3TVY, ECO:0007829|PDB:3TW0}
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 212-584 IN COMPLEX WITH
RP   MAGNESIUM.
RX   PubMed=23695252; DOI=10.1107/S0907444913004642;
RA   Krishnan V., Dwivedi P., Kim B.J., Samal A., Macon K., Ma X., Mishra A.,
RA   Doran K.S., Ton-That H., Narayana S.V.;
RT   "Structure of Streptococcus agalactiae tip pilin GBS104: a model for GBS
RT   pili assembly and host interactions.";
RL   Acta Crystallogr. D 69:1073-1089(2013).
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DR   EMBL; AE009948; AAM99541.1; -; Genomic_DNA.
DR   RefSeq; NP_687669.1; NC_004116.1.
DR   RefSeq; WP_001868236.1; NC_004116.1.
DR   PDB; 3TVY; X-ray; 2.00 A; A=212-584.
DR   PDB; 3TW0; X-ray; 2.00 A; A/B/C/D=212-581.
DR   PDB; 3TXA; X-ray; 2.62 A; A=48-729.
DR   PDBsum; 3TVY; -.
DR   PDBsum; 3TW0; -.
DR   PDBsum; 3TXA; -.
DR   AlphaFoldDB; Q8E0S5; -.
DR   SMR; Q8E0S5; -.
DR   STRING; 208435.SAG0649; -.
DR   KEGG; sag:SAG0649; -.
DR   PATRIC; fig|208435.3.peg.651; -.
DR   HOGENOM; CLU_323622_0_0_9; -.
DR   OrthoDB; 1744455at2; -.
DR   Proteomes; UP000000821; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd00198; vWFA; 1.
DR   Gene3D; 2.60.40.2110; -; 1.
DR   Gene3D; 3.30.70.1830; -; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR   InterPro; IPR049319; GBS104-like_Ig.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR041033; Prealbumin-like.
DR   InterPro; IPR002035; VWF_A.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   Pfam; PF21426; GBS104-like_Ig; 1.
DR   Pfam; PF17802; SpaA; 2.
DR   Pfam; PF00092; VWA; 1.
DR   SMART; SM00327; VWA; 1.
DR   SUPFAM; SSF49478; Cna protein B-type domain; 1.
DR   SUPFAM; SSF53300; vWA-like; 1.
DR   PROSITE; PS50234; VWFA; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:3TVY, ECO:0007829|PDB:3TW0};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Metal-binding {ECO:0007829|PDB:3TVY, ECO:0007829|PDB:3TW0};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000821};
KW   Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           25..890
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5038475134"
FT   TRANSMEM        863..885
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          223..385
FT                   /note="VWFA"
FT                   /evidence="ECO:0000259|PROSITE:PS50234"
FT   BINDING         229
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:3TVY"
FT   BINDING         231
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:3TVY, ECO:0007829|PDB:3TXA"
FT   BINDING         231
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:3TW0"
FT   BINDING         233
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:3TVY, ECO:0007829|PDB:3TXA"
FT   BINDING         233
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:3TW0"
FT   BINDING         354
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:3TW0"
FT   BINDING         384
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:3TVY, ECO:0007829|PDB:3TXA"
SQ   SEQUENCE   890 AA;  99447 MW;  AD9B7689083CB34F CRC64;
     MKKRQKIWRG LSVTLLILSQ IPFGILVQGE TQDTNQALGK VIVKKTGDNA TPLGKATFVL
     KNDNDKSETS HETVEGSGEA TFENIKPGDY TLREETAPIG YKKTDKTWKV KVADNGATII
     EGMDADKAEK RKEVLNAQYP KSAIYEDTKE NYPLVNVEGS KVGEQYKALN PINGKDGRRE
     IAEGWLSKKI TGVNDLDKNK YKIELTVEGK TTVETKELNQ PLDVVVLLDN SNSMNNERAN
     NSQRALKAGE AVEKLIDKIT SNKDNRVALV TYASTIFDGT EATVSKGVAD QNGKALNDSV
     SWDYHKTTFT ATTHNYSYLN LTNDANEVNI LKSRIPKEAE HINGDRTLYQ FGATFTQKAL
     MKANEILETQ SSNARKKLIF HVTDGVPTMS YAINFNPYIS TSYQNQFNSF LNKIPDRSGI
     LQEDFIINGD DYQIVKGDGE SFKLFSDRKV PVTGGTTQAA YRVPQNQLSV MSNEGYAINS
     GYIYLYWRDY NWVYPFDPKT KKVSATKQIK THGEPTTLYF NGNIRPKGYD IFTVGIGVNG
     DPGATPLEAE KFMQSISSKT ENYTNVDDTN KIYDELNKYF KTIVEEKHSI VDGNVTDPMG
     EMIEFQLKNG QSFTHDDYVL VGNDGSQLKN GVALGGPNSD GGILKDVTVT YDKTSQTIKI
     NHLNLGSGQK VVLTYDVRLK DNYISNKFYN TNNRTTLSPK SEKEPNTIRD FPIPKIRDVR
     EFPVLTISNQ KKMGEVEFIK VNKDKHSESL LGAKFQLQIE KDFSGYKQFV PEGSDVTTKN
     DGKIYFKALQ DGNYKLYEIS SPDGYIEVKT KPVVTFTIQN GEVTNLKADP NANKNQIGYL
     EGNGKHLITN TPKRPPGVFP KTGGIGTIVY ILVGSTFMIL TICSFRRKQL
//