Reviewed, UniProtKB/Swiss-Prot Q8E0N5 (LDHD_STRA5)

Names and origin

Protein names

Recommended name:
    D-lactate dehydrogenase
      Short name=D-LDH
    EC=1.1.1.28
Alternative name(s):
    D-specific D-2-hydroxyacid dehydrogenase

Gene names

Name:	ldhD
Synonyms:	ldhA
Ordered Locus Names:	SAG0695

Organism

Streptococcus agalactiae serotype V [Complete proteome] [HAMAP]

Taxonomic identifier

216466 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	D-lactate dehydrogenase		PRO_0000075969
Regions
	Nucleotide binding	155 – 156	2	NAD By similarity
	Nucleotide binding	233 – 235	3	NAD By similarity
	Nucleotide binding	296 – 299	4	NAD By similarity
Sites
	Active site	235	1	By similarity
	Active site	264	1	By similarity
	Active site	296	1	Proton donor By similarity
	Binding site	259	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8E0N5-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1F1589D42E3C9BF5	FASTA	330	36,560
	10 20 30 40 50 60 MKLKVFNVRE EEATLAQDWA NRNHVELSMS EGPLTLETVN EVEGFDGIAN AQIEPLDDAI 70 80 90 100 110 120 YPLLKEMGIK QIAQRSAGVD MYNLELAKQH GIIISNVPSY SPESIAEFTV TIALNLIRKV 130 140 150 160 170 180 ELIRANVREQ NFSWTLPIRG RVLGNMTVAI IGTGRIGLAT AKIFKGFGCR VIGYDIYHNP 190 200 210 220 230 240 MADGILEYVN SVEEAVEEAD LVSLHMPPTA ENTHLFNLDM FKQFKKGAIL MNMARGALVE 250 260 270 280 290 300 TKDLLEALDQ GLLEGAGIDT YEFEGPYIPK NCQGQDISDK DFLRLINHPK VIYTPHAAYY 310 320 330 TDEAVKNLVE GALNACVEVI ETGTTTTKVN

References

[1]

"Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae." Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S.N., Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D., Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F. , Madupu R., Lewis M.R., Radune D., Fedorova N.B., Scanlan D., Khouri H.M., Mulligan S., Carty H.A., Cline R.T., Van Aken S.E., Gill J., Scarselli M., Mora M., Iacobini E.T., Brettoni C., Galli G., Mariani M., Vegni F., Maione D., Rinaudo D., Rappuoli R., Telford J.L., Kasper D.L., Grandi G., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:12391-12396(2002) [PubMed: 12200547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-611 / 2603 V/R / Serotype V.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009948 Genomic DNA. Translation: AAM99582.1.
RefSeq	NP_687710.1.
3D structure databases
HSSP	HSSP built from PDB template 1DXY based on UniProtKB P17584.
ModBase	Search...
Genome annotation databases
GeneID	1013499.
GenomeReviews	Gene locus SAG0695 in contig AE009948_GR.
KEGG	sag:SAG0695.
TIGR	SAG0695.
Phylogenomic databases
HOGENOM	Q8E0N5.
OMA	KWGERAS.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_STRA5

Accession

Primary (citable) accession number: Q8E0N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)