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Q8E0G6 (DDL_STRA5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:SAG0767
OrganismStreptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R) [Complete proteome] [HAMAP]
Taxonomic identifier208435 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177883

Regions

Domain132 – 334203ATP-grasp
Nucleotide binding162 – 21756ATP By similarity

Sites

Metal binding2881Magnesium or manganese 1 By similarity
Metal binding3011Magnesium or manganese 1 By similarity
Metal binding3011Magnesium or manganese 2 By similarity
Metal binding3031Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8E0G6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 619B27BA8267F7E1

FASTA34839,068
        10         20         30         40         50         60 
MSKETLILLY GGRSAEREVS VLSAESVMRA INYDKFFVKT YFITQVGQFI KTQEFDEMPS 

        70         80         90        100        110        120 
SDEKLMTNQT VDLDKMVRPS DIYDDNAIVF PVLHGPMGED GSIQGFLEVL RMPYVGTNIL 

       130        140        150        160        170        180 
SSSVAMDKIT TKQVLATVGV PQVAYQTYFE GDDLEHAIKL SLETLSFPIF VKPANMGSSV 

       190        200        210        220        230        240 
GISKATDESS LRSAIDLALK YDSRILIEQG VTAREIEVGI LGNNDVKTTF PGEVVKDVDF 

       250        260        270        280        290        300 
YDYDAKYIDN KITMDIPAKV DEATMEAMRQ YASKAFKAIG ACGLSRCDFF LTKDGQIFLN 

       310        320        330        340 
ELNTMPGFTQ WSMYPLLWEN MGLTYSDLIE KLVMLAKEMF EKRESHLI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009948 Genomic DNA. Translation: AAM99654.1.
RefSeqNP_687782.1. NC_004116.1.

3D structure databases

ProteinModelPortalQ8E0G6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208435.SAG0767.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM99654; AAM99654; SAG0767.
GeneID1013571.
KEGGsag:SAG0767.
PATRIC19628366. VBIStrAga72745_0774.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycSAGA208435:GHVY-858-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_STRA5
AccessionPrimary (citable) accession number: Q8E0G6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways