Reviewed, UniProtKB/Swiss-Prot Q8E069 (MURA2_STRA5)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Synonyms:	murA-2
Ordered Locus Names:	SAG0866

Organism

Streptococcus agalactiae serotype V [Complete proteome] [HAMAP]

Taxonomic identifier

216466 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231276
Sites
	Active site	120	1	Proton donor By similarity
	Binding site	120	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8E069-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C1FFA1F8E35102B8	FASTA	423	45,242
	10 20 30 40 50 60 MDKIIVEGGQ TQLQGQVVIE GAKNAVLPLL AATILPSQGK TLLTNVPILS DVFTMNNVVR 70 80 90 100 110 120 GLDIQVDFNC DKKEILVDAS GDILDVAPYE FVSQMRASIV VLGPILARNG HAKVSMPGGC 130 140 150 160 170 180 TIGSRPIDLH LKGLEAMGAT ITQNGGDITA QAEKLKGANI YMDFPSVGAT QNLMMAATLA 190 200 210 220 230 240 SGTTTIENAA REPEIVDLAQ LLNKMGAKVK GAGTETLTII GVDALHGTEH DVVQDRIEAG 250 260 270 280 290 300 TFMVAAAMTS GNVLVKDAIW EHNRPLISKL MEMGVEVSEE EDGIRVKADT KKLKPVTVKT 310 320 330 340 350 360 LPHPGFPTDM QAQFTALMAV VNGESTMIET VFENRFQHLE EMRRMGLQTE ILRDTAMIHG 370 380 390 400 410 420 GRALQGAPVM STDLRASAAL ILAGMVAQGQ TVVGQLTHLD RGYYQFHEKL AALGANIKRV SEA

References

[1]

"Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae." Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S.N., Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D., Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F. , Madupu R., Lewis M.R., Radune D., Fedorova N.B., Scanlan D., Khouri H.M., Mulligan S., Carty H.A., Cline R.T., Van Aken S.E., Gill J., Scarselli M., Mora M., Iacobini E.T., Brettoni C., Galli G., Mariani M., Vegni F., Maione D., Rinaudo D., Rappuoli R., Telford J.L., Kasper D.L., Grandi G., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:12391-12396(2002) [PubMed: 12200547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-611 / 2603 V/R / Serotype V.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009948 Genomic DNA. Translation: AAM99752.1.
RefSeq	NP_687880.1.
3D structure databases
SMR	Q8E069. Positions 1-421.
ModBase	Search...
Genome annotation databases
GeneID	1013670.
GenomeReviews	Gene locus SAG0866 in contig AE009948_GR.
KEGG	sag:SAG0866.
TIGR	SAG0866.
Phylogenomic databases
HOGENOM	HBG482701.
OMA	VSRVYHL.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR020683. Ankyrin_rpt-contain_dom. IPR001986. EPSP_synthase_core. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 1 hit.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_STRA5

Accession

Primary (citable) accession number: Q8E069

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)