Q8DW19 (PTC_STRMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putrescine carbamoyltransferase
Short name=PTC
Short name=PTCase
EC=2.1.3.6

Alternative name(s):
Putrescine transcarbamoylase
Putrescine transcarbamylase

Gene names

Name:	ptcA
Synonyms:	aguB, arcB
Ordered Locus Names:	SMU_262

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine Probable. Is involved in the degradation pathway of the polyamine agmatine. Ref.1
Catalytic activity	Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine. HAMAP MF_02102
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from N-carbamoylputrescine (transferase route): step 1/1. HAMAP MF_02102
Subunit structure	Homotrimer By similarity. HAMAP MF_02102
Subcellular location	Cytoplasm By similarity HAMAP MF_02102.
Induction	Up-regulated by agmatine. Expressed under the control of carbohydrate catabolite repression. Ref.1
Sequence similarities	Belongs to the ATCase/OTCase family. PTCase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	polyamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: InterPro putrescine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Putrescine carbamoyltransferase HAMAP MF_02102		PRO_0000113034
Regions
	Region	54 – 58	5	Carbamoyl phosphate binding By similarity
	Region	270 – 273	4	Putrescine binding By similarity
Sites
	Binding site	105	1	Carbamoyl phosphate By similarity
	Binding site	132	1	Carbamoyl phosphate By similarity
	Site	29	1	Important for structural integrity By similarity
	Site	145	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8DW19 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A51CA727DC5BA8CC	FASTA	339	38,519
	10 20 30 40 50 60 MMKKTDYITT EDFSKEELLK LVDLSLKIKA CIKNGYYPPL LEHKSLGMIF QQTSTRTRVS 70 80 90 100 110 120 FETAMSQLGG HAQYLAPGQI QLGGHETIED TSTVLSRLDD ILMARVERHQ SVVDLARCAS 130 140 150 160 170 180 IPVINGMSDY NHPTQELGDL CTMIEHLPAG KKLEDCKVVF VGDATQVCFS LALITTKMGM 190 200 210 220 230 240 EFVHFGPKGF QLNDMHKEKL DKICERSGGK YTVTDNEDAI EGADFLYTDV WYGLYEAELS 250 260 270 280 290 300 EEERMQIFFP KYQVDSQMMA KAGADCKFMH CLPATRGEEI TDEVMDGPHS ICFDEAENRL 310 320 330 TSIRGLLVYL LRDYREKNPY DLVKQEKAKE ELETFLKPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of an agmatine deiminase gene cluster in Streptococcus mutans UA159." Griswold A.R., Chen Y.-Y.M., Burne R.A. J. Bacteriol. 186:1902-1904(2004) [PubMed: 14996823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN AGMATINE CATABOLISM, INDUCTION. Strain: ATCC 700610 / UA159 / Serotype c.
[2]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.
[3]	"Retrieving sequences of enzymes experimentally characterized but erroneously annotated: the case of the putrescine carbamoyltransferase." Naumoff D.G., Xu Y., Glansdorff N., Labedan B. BMC Genomics 5:52-52(2004) [PubMed: 15287962] [Abstract] Cited for: REANNOTATION AS A PTCASE.
[4]	"The difficulty of annotating genes: the case of putrescine carbamoyltransferase." Naumoff D.G., Xu Y., Stalon V., Glansdorff N., Labedan B. Microbiology 150:3908-3911(2004) [PubMed: 15583144] [Abstract] Cited for: GENE NAME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BK004003 Genomic DNA. Translation: DAA04556.1. AE014133 Genomic DNA. Translation: AAN58031.1.
RefSeq	NP_720725.1. NC_004350.2.
3D structure databases
ProteinModelPortal	Q8DW19.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000014083; EBSTRP00000013565; EBSTRG00000014083.
GeneID	1027850.
GenomeReviews	Gene locus SMU_262 in contig AE014133_GR.
KEGG	smu:SMU_262.
NMPDR	fig|210007.1.peg.238.
PATRIC	19662785. VBIStrMut61772_0228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027080.
HOGENOM	HBG579429.
OMA	LGMIFQQ.
ProtClustDB	PRK02255.
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_262-MONOMER.
Family and domain databases
HAMAP	MF_02102. PTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024903. PtcA. [Graphical view]
KO	K13252.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTC_STRMU

Accession

Primary (citable) accession number: Q8DW19
Secondary accession number(s): Q6IFZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program