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Q8DW15

- GSHAB_STRMU

UniProt

Q8DW15 - GSHAB_STRMU

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Protein
Glutathione biosynthesis bifunctional protein GshAB
Gene
gshAB, gshF, SMU_267c
Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi696 – 6961Magnesium or manganese 1 By similarity
Metal bindingi717 – 7171Magnesium or manganese 1 By similarity
Metal bindingi717 – 7171Magnesium or manganese 2 By similarity
Metal bindingi719 – 7191Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi516 – 57459ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-281-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name:
GCS-GS
Including the following 2 domains:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GS
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshAB
Synonyms:gshF
Ordered Locus Names:SMU_267c
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Glutathione biosynthesis bifunctional protein GshABUniRule annotation
PRO_0000192560Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi210007.SMU.267c.

Structurei

3D structure databases

ProteinModelPortaliQ8DW15.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini489 – 752264ATP-grasp
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 333333Glutamate--cysteine ligaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG1181.
KOiK01919.
OMAiHVEYVKN.
OrthoDBiEOG6BKJ7H.
PhylomeDBiQ8DW15.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DW15-1 [UniParc]FASTAAdd to Basket

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MHINQLLQHA NSDLPLLQAN FGLERESLRI NKTNHRLAQT PHPTALGSRQ    50
FHPYIQTDYS ESQMELITPV AHSSKEVLRF LGAITDVAER SIDQNQYLWP 100
LSMPPQITED EIEIAQLEDD FEFSYRQYLD KKYGKILQSI SGIHYNMELG 150
ADLMNELFEL SGYQSFIDFK NDLYLKVAQN FLNYRWFLTY LYGASPLAEK 200
GFLNEELSQT VRSIRNSHLG YVNTDDIKVP FDSLENYISS IEHYVKSGAL 250
SAEKEFYSAV RLRGSKHNRD YLTKGITYLE FRCFDLNPFN NRGITQETID 300
SVHLFILAML WLDTPKKLNQ ALDKAQKLND KIALSHPLEK LPKENSASLI 350
IEAMEALIKH FKLPSYYDDL LIAIKKQVEN PKLTLSGRLF EHIKHASLEH 400
FGQKKGQDYH NYAWQNYYAL KGYENMELST QMLLFDTIQK GIHFEILDEN 450
DQFLKLWHND HIEYVKNGNM TSKDNYVIPL AMANKVVTKK ILRENGYPVP 500
AGAEFDNKDE ALRYYSQIKN KPIVVKPKTT NFGLGISIFE TAASHNDYEK 550
ALDIAFIEDY SVLVEEFIPG TEYRFFILDG KCEAVLLRVA ANVVGDGHST 600
VRQLVAQKNR DPLRGREHRS PLEIIDLGDI ELLMLQQEGY TLEDILPKGK 650
KVNLRGNSNI STGGDSIDVT ETMDPSYKQL AANMATAMGA WVCGVDLIIP 700
DTNLKASKGK PNCTCIELNF NPSMYMHTYC YQGPGQVITG KILAKLFPEI 750
STKI 754
Length:754
Mass (Da):86,187
Last modified:March 1, 2003 - v1
Checksum:i103479A8536A10CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014133 Genomic DNA. Translation: AAN58035.1.
RefSeqiNP_720729.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58035; AAN58035; SMU_267c.
GeneIDi1027852.
KEGGismu:SMU_267c.
PATRICi19662795. VBIStrMut61772_0233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014133 Genomic DNA. Translation: AAN58035.1 .
RefSeqi NP_720729.1. NC_004350.2.

3D structure databases

ProteinModelPortali Q8DW15.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 210007.SMU.267c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN58035 ; AAN58035 ; SMU_267c .
GeneIDi 1027852.
KEGGi smu:SMU_267c.
PATRICi 19662795. VBIStrMut61772_0233.

Phylogenomic databases

eggNOGi COG1181.
KOi K01919.
OMAi HVEYVKN.
OrthoDBi EOG6BKJ7H.
PhylomeDBi Q8DW15.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci SMUT210007:GC7Z-281-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.

Entry informationi

Entry nameiGSHAB_STRMU
AccessioniPrimary (citable) accession number: Q8DW15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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