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Q8DW15

- GSHAB_STRMU

UniProt

Q8DW15 - GSHAB_STRMU

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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi696 – 6961Magnesium or manganese 1UniRule annotation
Metal bindingi717 – 7171Magnesium or manganese 1UniRule annotation
Metal bindingi717 – 7171Magnesium or manganese 2UniRule annotation
Metal bindingi719 – 7191Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi516 – 57459ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-281-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:SMU_267c
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Glutathione biosynthesis bifunctional protein GshABPRO_0000192560Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi210007.SMU.267c.

Structurei

3D structure databases

ProteinModelPortaliQ8DW15.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini489 – 752264ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 333333Glutamate--cysteine ligaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1181.
KOiK01919.
OMAiHVEYVKN.
OrthoDBiEOG6BKJ7H.
PhylomeDBiQ8DW15.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DW15-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHINQLLQHA NSDLPLLQAN FGLERESLRI NKTNHRLAQT PHPTALGSRQ
60 70 80 90 100
FHPYIQTDYS ESQMELITPV AHSSKEVLRF LGAITDVAER SIDQNQYLWP
110 120 130 140 150
LSMPPQITED EIEIAQLEDD FEFSYRQYLD KKYGKILQSI SGIHYNMELG
160 170 180 190 200
ADLMNELFEL SGYQSFIDFK NDLYLKVAQN FLNYRWFLTY LYGASPLAEK
210 220 230 240 250
GFLNEELSQT VRSIRNSHLG YVNTDDIKVP FDSLENYISS IEHYVKSGAL
260 270 280 290 300
SAEKEFYSAV RLRGSKHNRD YLTKGITYLE FRCFDLNPFN NRGITQETID
310 320 330 340 350
SVHLFILAML WLDTPKKLNQ ALDKAQKLND KIALSHPLEK LPKENSASLI
360 370 380 390 400
IEAMEALIKH FKLPSYYDDL LIAIKKQVEN PKLTLSGRLF EHIKHASLEH
410 420 430 440 450
FGQKKGQDYH NYAWQNYYAL KGYENMELST QMLLFDTIQK GIHFEILDEN
460 470 480 490 500
DQFLKLWHND HIEYVKNGNM TSKDNYVIPL AMANKVVTKK ILRENGYPVP
510 520 530 540 550
AGAEFDNKDE ALRYYSQIKN KPIVVKPKTT NFGLGISIFE TAASHNDYEK
560 570 580 590 600
ALDIAFIEDY SVLVEEFIPG TEYRFFILDG KCEAVLLRVA ANVVGDGHST
610 620 630 640 650
VRQLVAQKNR DPLRGREHRS PLEIIDLGDI ELLMLQQEGY TLEDILPKGK
660 670 680 690 700
KVNLRGNSNI STGGDSIDVT ETMDPSYKQL AANMATAMGA WVCGVDLIIP
710 720 730 740 750
DTNLKASKGK PNCTCIELNF NPSMYMHTYC YQGPGQVITG KILAKLFPEI

STKI
Length:754
Mass (Da):86,187
Last modified:March 1, 2003 - v1
Checksum:i103479A8536A10CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014133 Genomic DNA. Translation: AAN58035.1.
RefSeqiNP_720729.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58035; AAN58035; SMU_267c.
GeneIDi1027852.
KEGGismu:SMU_267c.
PATRICi19662795. VBIStrMut61772_0233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014133 Genomic DNA. Translation: AAN58035.1 .
RefSeqi NP_720729.1. NC_004350.2.

3D structure databases

ProteinModelPortali Q8DW15.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 210007.SMU.267c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN58035 ; AAN58035 ; SMU_267c .
GeneIDi 1027852.
KEGGi smu:SMU_267c.
PATRICi 19662795. VBIStrMut61772_0233.

Phylogenomic databases

eggNOGi COG1181.
KOi K01919.
OMAi HVEYVKN.
OrthoDBi EOG6BKJ7H.
PhylomeDBi Q8DW15.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci SMUT210007:GC7Z-281-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.

Entry informationi

Entry nameiGSHAB_STRMU
AccessioniPrimary (citable) accession number: Q8DW15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3