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Q8DVD3

- SYI_STRMU

UniProt

Q8DVD3 - SYI_STRMU

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Protein
Isoleucine--tRNA ligase
Gene
ileS, SMU_558
Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylate By similarity
Binding sitei598 – 5981ATP By similarity
Metal bindingi888 – 8881Zinc By similarity
Metal bindingi891 – 8911Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-550-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SMU_558
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098478Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi210007.SMU.558.

Structurei

3D structure databases

ProteinModelPortaliQ8DVD3.
SMRiQ8DVD3. Positions 1-918.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.
PhylomeDBiQ8DVD3.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DVD3-1 [UniParc]FASTAAdd to Basket

« Hide

MKLKETLNLG KTAFPMRAGL PNKEPQWQKQ WDEANIYAKR QELNANKPAF    50
FLHDGPPYAN GNIHVGHALN KISKDIIIRS KSMSGFRAPY IPGWDTHGLP 100
IEQVLAKKGV KRKEIDLADY LDMCRQYALS QVDKQRQDFK RLGVSGDWEN 150
PYITLVPKYE AAQIRVFGAM ADKGYIYHGA KPVYWSWSSE SALAEAEIEY 200
HDIDSTSLYY ANRVKDGKDI LDTDTYIVVW TTTPFTITAS RGLTVGPDID 250
YVVVKPANDE RKFLVAQALL SELAERFAWD NPQVLATHKG IELDRIVTIH 300
PWDDNVEELV MNGDHVTLDS GTGIVHTAPG FGEDDYNVGV KYGLDVVVTV 350
NERGIMMENA GPDFEGQFYD KVLPTVKEKL GDLLLASEVI THSYPFDWRT 400
KKPIIWRAVP QWFASVSKFR QDILDEIDKV HFYPAWGKTR LYNMIRDRGD 450
WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHIANLFEEH GSVIWWQREA 500
KDLLPEGFTH PGSPNGEFTK ENDIMDVWFD SGSSWNGVLN TREDLGYPAD 550
LYLEGSDQYR GWFNSSLITS VAVNGHAPYK SVLSQGFVLD GKGEKMSKSK 600
GNIISPNDVA KQYGAEILRL WVASVDTDSD VRVSMEILGQ VSETYRKIRN 650
TLRFLIANTT DFNPYINKIA FEDLRSVDKY MLIKFNQLVS VINRAYSHYD 700
FMTIYKAVVN FVTVDLSAFY LDFAKDVVYI EAADDLARRQ MQTVFYEILV 750
NITKLLTPIL PHTSEEIWSY LEHEEEAFVQ LAEMPEAQEF ANQDEILDTW 800
SAFMSLRDQA QKALEEARNA KIIGKSLEAH LTVYASEEVK TLLTALDSNI 850
AQLLIVSQLT VTQEQAPKNA LVFEDVAFSV EHARGHVCDR CRRIDETVKE 900
RPYHVTICNH CAAIVENHFP EAVRQGFESK 930
Length:930
Mass (Da):105,853
Last modified:March 1, 2003 - v1
Checksum:i40024D46A4BAE8C2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014133 Genomic DNA. Translation: AAN58300.1.
RefSeqiNP_720994.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58300; AAN58300; SMU_558.
GeneIDi1028027.
KEGGismu:SMU_558.
PATRICi19663321. VBIStrMut61772_0493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014133 Genomic DNA. Translation: AAN58300.1 .
RefSeqi NP_720994.1. NC_004350.2.

3D structure databases

ProteinModelPortali Q8DVD3.
SMRi Q8DVD3. Positions 1-918.
ModBasei Search...

Protein-protein interaction databases

STRINGi 210007.SMU.558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN58300 ; AAN58300 ; SMU_558 .
GeneIDi 1028027.
KEGGi smu:SMU_558.
PATRICi 19663321. VBIStrMut61772_0493.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.
PhylomeDBi Q8DVD3.

Enzyme and pathway databases

BioCyci SMUT210007:GC7Z-550-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.

Entry informationi

Entry nameiSYI_STRMU
AccessioniPrimary (citable) accession number: Q8DVD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi