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Q8DVD3

- SYI_STRMU

UniProt

Q8DVD3 - SYI_STRMU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
    Binding sitei598 – 5981ATPUniRule annotation
    Metal bindingi888 – 8881ZincUniRule annotation
    Metal bindingi891 – 8911ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSMUT210007:GC7Z-550-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:SMU_558
    OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
    Taxonomic identifieri210007 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000002512: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 930930Isoleucine--tRNA ligasePRO_0000098478Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi210007.SMU.558.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8DVD3.
    SMRiQ8DVD3. Positions 1-918.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi595 – 5995"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.
    PhylomeDBiQ8DVD3.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8DVD3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLKETLNLG KTAFPMRAGL PNKEPQWQKQ WDEANIYAKR QELNANKPAF    50
    FLHDGPPYAN GNIHVGHALN KISKDIIIRS KSMSGFRAPY IPGWDTHGLP 100
    IEQVLAKKGV KRKEIDLADY LDMCRQYALS QVDKQRQDFK RLGVSGDWEN 150
    PYITLVPKYE AAQIRVFGAM ADKGYIYHGA KPVYWSWSSE SALAEAEIEY 200
    HDIDSTSLYY ANRVKDGKDI LDTDTYIVVW TTTPFTITAS RGLTVGPDID 250
    YVVVKPANDE RKFLVAQALL SELAERFAWD NPQVLATHKG IELDRIVTIH 300
    PWDDNVEELV MNGDHVTLDS GTGIVHTAPG FGEDDYNVGV KYGLDVVVTV 350
    NERGIMMENA GPDFEGQFYD KVLPTVKEKL GDLLLASEVI THSYPFDWRT 400
    KKPIIWRAVP QWFASVSKFR QDILDEIDKV HFYPAWGKTR LYNMIRDRGD 450
    WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHIANLFEEH GSVIWWQREA 500
    KDLLPEGFTH PGSPNGEFTK ENDIMDVWFD SGSSWNGVLN TREDLGYPAD 550
    LYLEGSDQYR GWFNSSLITS VAVNGHAPYK SVLSQGFVLD GKGEKMSKSK 600
    GNIISPNDVA KQYGAEILRL WVASVDTDSD VRVSMEILGQ VSETYRKIRN 650
    TLRFLIANTT DFNPYINKIA FEDLRSVDKY MLIKFNQLVS VINRAYSHYD 700
    FMTIYKAVVN FVTVDLSAFY LDFAKDVVYI EAADDLARRQ MQTVFYEILV 750
    NITKLLTPIL PHTSEEIWSY LEHEEEAFVQ LAEMPEAQEF ANQDEILDTW 800
    SAFMSLRDQA QKALEEARNA KIIGKSLEAH LTVYASEEVK TLLTALDSNI 850
    AQLLIVSQLT VTQEQAPKNA LVFEDVAFSV EHARGHVCDR CRRIDETVKE 900
    RPYHVTICNH CAAIVENHFP EAVRQGFESK 930
    Length:930
    Mass (Da):105,853
    Last modified:March 1, 2003 - v1
    Checksum:i40024D46A4BAE8C2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014133 Genomic DNA. Translation: AAN58300.1.
    RefSeqiNP_720994.1. NC_004350.2.

    Genome annotation databases

    EnsemblBacteriaiAAN58300; AAN58300; SMU_558.
    GeneIDi1028027.
    KEGGismu:SMU_558.
    PATRICi19663321. VBIStrMut61772_0493.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014133 Genomic DNA. Translation: AAN58300.1 .
    RefSeqi NP_720994.1. NC_004350.2.

    3D structure databases

    ProteinModelPortali Q8DVD3.
    SMRi Q8DVD3. Positions 1-918.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 210007.SMU.558.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN58300 ; AAN58300 ; SMU_558 .
    GeneIDi 1028027.
    KEGGi smu:SMU_558.
    PATRICi 19663321. VBIStrMut61772_0493.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.
    PhylomeDBi Q8DVD3.

    Enzyme and pathway databases

    BioCyci SMUT210007:GC7Z-550-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700610 / UA159.

    Entry informationi

    Entry nameiSYI_STRMU
    AccessioniPrimary (citable) accession number: Q8DVD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3