Q8DU17 (SPX_STRMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein spx

Gene names

Name:	spxA
Ordered Locus Names:	SMU_1142c

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity. HAMAP MF_01132
Subcellular location	Cytoplasm Probable HAMAP MF_01132.
Sequence similarities	Belongs to the ArsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 137

137

Regulatory protein spx HAMAP MF_01132

PRO_0000162574

Amino acid modifications

Disulfide bond

10 ↔ 13

Redox-active Potential

Secondary structure

1

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137

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

11 – 22

12

Beta strand

27 – 31

5

Turn

32 – 34

3

Helix

39 – 48

10

Turn

54 – 56

3

Helix

62 – 66

5

Helix

71 – 73

3

Helix

76 – 85

10

Helix

87 – 89

3

Beta strand

94 – 96

3

Helix

109 – 113

5

Sequences

	Sequence		Length	Mass (Da)
	Q8DU17 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 736821A9B10FCA1F	FASTA	137	15,968
	10 20 30 40 50 60 MVTLFLSPSC TSCRKARAWL NRHDVVFQEH NIMTSPLSRD ELLKILSYTE NGTEDIISTR 70 80 90 100 110 120 SKVFQKLDID VDELSVSELI NLISKNPSLL RRPIIMDNKR MQIGFNEDEI RAFLPRDYRK 130 QELRQATIRA EVEGEDD

References

[1]

"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE014133 Genomic DNA. Translation: AAN58834.1.

RefSeq

NP_721528.1. NC_004350.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3L78	X-ray	1.90	A	1-120	[»]

ProteinModelPortal

Q8DU17.

SMR

Q8DU17. Positions 1-116.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBSTRT00000012701; EBSTRP00000012183; EBSTRG00000012701.

GeneID

1029353.

GenomeReviews

Gene locus SMU_1142c in contig AE014133_GR.

KEGG

smu:SMU_1142c.

NMPDR

fig|210007.1.peg.1041.

PATRIC

19664383. VBIStrMut61772_1024.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000027616.

HOGENOM

HBG734370.

OMA

FTERNIF.

ProtClustDB

PRK01655.

Enzyme and pathway databases

BioCyc

SMUT210007:SMU_1142C-MONOMER.

Family and domain databases

HAMAP

MF_01132. SpxA.
[Tree]

InterPro

IPR006660. Arsenate_reductase-like.
IPR012336. Thioredoxin-like_fold.
IPR023731. Tscrpt_reg_Spx.
IPR006504. Tscrpt_reg_Spx/MgsR.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

KO

K00537.

Pfam

PF03960. ArsC. 1 hit.
[Graphical view]

SUPFAM

SSF52833. Thiordxn-like_fd. 1 hit.

TIGRFAMs

TIGR01617. ArsC_related. 1 hit.

PROSITE

PS51353. ARSC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SPX_STRMU

Accession

Primary (citable) accession number: Q8DU17

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program