Q8DTX6 (K6PF_STRMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Synonyms:	pfk
Ordered Locus Names:	SMU_1191

Organism

Streptococcus mutans serotype c (strain ATCC 700610 / UA159) [Complete proteome] [HAMAP]

Taxonomic identifier

210007 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus mutans

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_0000111989
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
	Nucleotide binding	154 – 158	5	ATP By similarity
	Nucleotide binding	171 – 187	17	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Metal binding	185	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	187	1	Magnesium By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8DTX6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0D94A1020AEF61B4	FASTA	337	35,771
	10 20 30 40 50 60 MKRIAVLTSG GDAPGMNAAV RAVVRKAISE GMEVCGINRG YAGMVEGDIF PLDAKGVSNI 70 80 90 100 110 120 LSRGGTFLQS ARYPEFAKLE GQLKGIEQLK KHGIEGVVVI GGDGSYHGAM RLTEHGFPAV 130 140 150 160 170 180 GLPGTIDNDI VGTDYTIGFD TAVNTATDAL DKIRDTSFSH GRTFVVEVMG RNAGDIALWS 190 200 210 220 230 240 GIAAGADQII IPEEPYDIKE VVANVKNGYL SKSKNHHLIV LAEGVMHGEE FAAQMKEAGD 250 260 270 280 290 300 NSDLRVTNLG HILRGGAPTP RDRVIASWMG AHAVELLREG KGGLAIGIQN EELVEHPILG 310 320 330 SAEDGALFSL TEQGEIVVNN PHKARLDFAA LNRDLSN

References

[1]

"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014133 Genomic DNA. Translation: AAN58880.1.
RefSeq	NP_721574.1. NC_004350.2.
3D structure databases
ProteinModelPortal	Q8DTX6.
SMR	Q8DTX6. Positions 1-298.
ModBase	Search...
Protein-protein interaction databases
STRING	210007.SMU.1191.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN58880; AAN58880; SMU_1191.
GeneID	1028488.
KEGG	smu:SMU_1191.
PATRIC	19664471. VBIStrMut61772_1068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0205.
KO	K00850.
OMA	EFKTVEG.
ProtClustDB	PRK03202.
Enzyme and pathway databases
BioCyc	SMUT210007:GC7Z-1130-MONOMER.
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_STRMU

Accession

Primary (citable) accession number: Q8DTX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program