Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-isopropylmalate dehydratase small subunit

Gene

leuD

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-1303-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase small subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuDUniRule annotation
Ordered Locus Names:SMU_1381
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000002512 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1961963-isopropylmalate dehydratase small subunitPRO_0000141893Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi210007.SMU_1381.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 1410Combined sources
Helixi21 – 244Combined sources
Helixi27 – 315Combined sources
Helixi39 – 413Combined sources
Helixi44 – 474Combined sources
Beta strandi48 – 503Combined sources
Helixi60 – 623Combined sources
Helixi64 – 663Combined sources
Beta strandi71 – 744Combined sources
Beta strandi76 – 783Combined sources
Helixi85 – 9410Combined sources
Beta strandi98 – 1025Combined sources
Helixi106 – 1138Combined sources
Turni114 – 1163Combined sources
Beta strandi118 – 1214Combined sources
Helixi124 – 1318Combined sources
Beta strandi138 – 1425Combined sources
Turni143 – 1464Combined sources
Beta strandi147 – 1504Combined sources
Beta strandi153 – 1564Combined sources
Helixi161 – 1688Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HCUX-ray2.10A1-196[»]
ProteinModelPortaliQ8DTG5.
SMRiQ8DTG5. Positions 1-172.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8DTG5.

Family & Domainsi

Sequence similaritiesi

Belongs to the LeuD family. LeuD type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105MQS. Bacteria.
COG0066. LUCA.
KOiK01704.
OMAiAFTTHTG.
PhylomeDBiQ8DTG5.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
HAMAPiMF_01031. LeuD_type1. 1 hit.
InterProiIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00171. leuD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8DTG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEFTIYTGT TVPLMNDNID TDQILPKQFL KLIDKKGFGK YLMYEWRYLD
60 70 80 90 100
NNYTENPDFI FNQPEYREAS ILITGDNFGA GSSREHAAWA LADYGFKVIV
110 120 130 140 150
AGSFGDIHYN NDLNNGILPI IQPKEVRDKL AKLKPTDEVT VNLFEQKIYS
160 170 180 190
PVGDFSFDID GEWKHKLLNG LDDIGITLQY EDLIAQYEQN RPSYWH
Length:196
Mass (Da):22,689
Last modified:March 1, 2003 - v1
Checksum:iE27F1A35FA9ED7FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014133 Genomic DNA. Translation: AAN59048.1.
RefSeqiNP_721742.1. NC_004350.2.
WP_002262705.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN59048; AAN59048; SMU_1381.
GeneIDi1028650.
KEGGismu:SMU_1381.
PATRICi19664799. VBIStrMut61772_1228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014133 Genomic DNA. Translation: AAN59048.1.
RefSeqiNP_721742.1. NC_004350.2.
WP_002262705.1. NC_004350.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HCUX-ray2.10A1-196[»]
ProteinModelPortaliQ8DTG5.
SMRiQ8DTG5. Positions 1-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi210007.SMU_1381.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN59048; AAN59048; SMU_1381.
GeneIDi1028650.
KEGGismu:SMU_1381.
PATRICi19664799. VBIStrMut61772_1228.

Phylogenomic databases

eggNOGiENOG4105MQS. Bacteria.
COG0066. LUCA.
KOiK01704.
OMAiAFTTHTG.
PhylomeDBiQ8DTG5.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.
BioCyciSMUT210007:GC7Z-1303-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8DTG5.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
HAMAPiMF_01031. LeuD_type1. 1 hit.
InterProiIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00171. leuD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLEUD_STRMU
AccessioniPrimary (citable) accession number: Q8DTG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.