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Q8DT66

- RNC_STRMU

UniProt

Q8DT66 - RNC_STRMU

Protein

Ribonuclease 3

Gene

rnc

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity.By similarity
    CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA Probable. Complements pre-crRNA and tracrRNA coprocessing defects in an rnc deletion in S.pyogenes strain 370 (PubMed:24270795).1 PublicationCurated

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

    Cofactori

    Mg2+.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471MagnesiumUniRule annotation
    Active sitei51 – 511UniRule annotation
    Metal bindingi120 – 1201MagnesiumUniRule annotation
    Active sitei123 – 1231UniRule annotation
    Metal bindingi123 – 1231MagnesiumUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonuclease III activity Source: UniProtKB-HAMAP
    3. rRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-HAMAP
    2. rRNA catabolic process Source: InterPro
    3. rRNA processing Source: UniProtKB-HAMAP
    4. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciSMUT210007:GC7Z-1420-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
    Alternative name(s):
    Ribonuclease IIIUniRule annotation
    Short name:
    RNase IIIUniRule annotation
    Gene namesi
    Name:rncUniRule annotation
    Ordered Locus Names:SMU_1514
    OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
    Taxonomic identifieri210007 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000002512: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 231231Ribonuclease 3PRO_0000228590Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi210007.SMU.1514.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8DT66.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 134134RNase IIIUniRule annotationAdd
    BLAST
    Domaini160 – 22970DRBMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
    Contains 1 RNase III domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0571.
    KOiK03685.
    OMAiYGFQEAR.
    OrthoDBiEOG6T1WVS.
    PhylomeDBiQ8DT66.

    Family and domain databases

    Gene3Di1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 1 hit.
    TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8DT66-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTLEKKLAE DFKIVFSDKE LLETAFTHTS YANEHRLLNI SHNERLEFLG    50
    DAVLQLTISH YLFDKYPQKA EGDLSKMRSM IVREESLAGF SRNCHFDRYI 100
    KLGKGEEKSG GRQRDTILGD LFEAFLGALL LDAGLKAVEA FLNQVVIPKV 150
    ENNNYERVTD YKTALQELLQ VDGDVLIDYE VLKESGPAHA KCFEVAVSMN 200
    HEKLSSGTGK SKKLAEQEAA KNALEKLQRG S 231
    Length:231
    Mass (Da):26,029
    Last modified:March 1, 2003 - v1
    Checksum:i2D8D6A8257791C0E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014133 Genomic DNA. Translation: AAN59165.1.
    RefSeqiNP_721859.1. NC_004350.2.

    Genome annotation databases

    EnsemblBacteriaiAAN59165; AAN59165; SMU_1514.
    GeneIDi1028756.
    KEGGismu:SMU_1514.
    PATRICi19665037. VBIStrMut61772_1347.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014133 Genomic DNA. Translation: AAN59165.1 .
    RefSeqi NP_721859.1. NC_004350.2.

    3D structure databases

    ProteinModelPortali Q8DT66.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 210007.SMU.1514.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN59165 ; AAN59165 ; SMU_1514 .
    GeneIDi 1028756.
    KEGGi smu:SMU_1514.
    PATRICi 19665037. VBIStrMut61772_1347.

    Phylogenomic databases

    eggNOGi COG0571.
    KOi K03685.
    OMAi YGFQEAR.
    OrthoDBi EOG6T1WVS.
    PhylomeDBi Q8DT66.

    Enzyme and pathway databases

    BioCyci SMUT210007:GC7Z-1420-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 1 hit.
    TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700610 / UA159.
    2. "Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems."
      Fonfara I., Le Rhun A., Chylinski K., Makarova K.S., Lecrivain A.L., Bzdrenga J., Koonin E.V., Charpentier E.
      Nucleic Acids Res. 42:2577-2590(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiRNC_STRMU
    AccessioniPrimary (citable) accession number: Q8DT66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3