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Q8DQB5

- Q8DQB5_STRR6

UniProt

Q8DQB5 - Q8DQB5_STRR6

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Protein
DNA topoisomerase 4 subunit B
Gene
parE, spr0756
Organism
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Status
Unreviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP By similarityUniRule annotation
Binding sitei51 – 511ATP By similarityUniRule annotation
Binding sitei78 – 781ATP By similarityUniRule annotation
Binding sitei344 – 3441ATP By similarityUniRule annotation
Metal bindingi433 – 4331Magnesium 1; catalytic By similarityUniRule annotation
Sitei458 – 4581Interaction with DNA By similarityUniRule annotation
Sitei461 – 4611Interaction with DNA By similarityUniRule annotation
Metal bindingi506 – 5061Magnesium 1; catalytic By similarityUniRule annotation
Metal bindingi506 – 5061Magnesium 2 By similarityUniRule annotation
Metal bindingi508 – 5081Magnesium 2 By similarityUniRule annotation
Sitei513 – 5131Interaction with DNA By similarityUniRule annotation
Sitei629 – 6291Interaction with DNA By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi118 – 1247ATP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
  2. chromosome segregation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, DNA-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPNE171101:GJC8-763-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit B
Gene namesi
Name:parEUniRule annotationImported
Ordered Locus Names:spr0756Imported
OrganismiStreptococcus pneumoniae (strain ATCC BAA-255 / R6)Imported
Taxonomic identifieri171101 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000586: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi171101.spr0756.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LP0X-ray1.95A1-226[»]
4LPBX-ray1.75A1-226[»]
4MOTX-ray1.75A1-226[»]
ProteinModelPortaliQ8DQB5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini427 – 541115Toprim By similarityUniRule annotation
Add
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation
Contains Toprim domain.SAAS annotations

Phylogenomic databases

eggNOGiCOG0187.
HOGENOMiHOG000075154.
KOiK02622.
OMAiREEFHYE.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_ATP-bd.
IPR005740. ParE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DQB5-1 [UniParc]FASTAAdd to Basket

« Hide

MSKKEININN YNDDAIQVLE GLDAVRKRPG MYIGSTDGAG LHHLVWEIVD    50
NAVDEALSGF GDRIDVTINK DGSLTVQDHG RGMPTGMHAM GIPTVEVIFT 100
ILHAGGKFGQ GGYKTSGGLH GVGSSVVNAL SSWLEVEITR DGAVYKQRFE 150
NGGKPVTTLK KIGTALKSKT GTKVTFMPDA TIFSTTDFKY NTISERLNES 200
AFLLKNVTLS LTDKRTDEAI EFHYENGVQD FVSYLNEDKE ILTPVLYFEG 250
EDNGFQVEVA LQYNDGFSDN ILSFVNNVRT KDGGTHETGL KSAITKVMND 300
YARKTGLLKE KDKNLEGSDY REGLAAVLSI LVPEEHLQFE GQTKDKLGSP 350
LARPVVDGIV ADKLTFFLME NGELASNLIR KAIKARDARE AARKARDESR 400
NGKKNKKDKG LLSGKLTPAQ SKNPAKNELY LVEGDSAGGS AKQGRDRKFQ 450
AILPLRGKVI NTAKAKMADI LKNEEINTMI YTIGAGVGAD FSIEDANYDK 500
IIIMTDADTD GAHIQTLLLT FFYRYMRPLV EAGHVYIALP PLYKMSKGKG 550
KKEEVAYAWT DGELEELRKQ FGKGATLQRY KGLGEMNADQ LWETTMNPET 600
RTLIRVTIED LARAERRVNV LMGDKVEPRR KWIEDNVKFT LEEATVF 647
Length:647
Mass (Da):71,665
Last modified:March 1, 2003 - v1
Checksum:i0AF7382C72772713
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007317 Genomic DNA. Translation: AAK99560.1.
PIRiD97966.
RefSeqiNP_358350.1. NC_003098.1.

Genome annotation databases

EnsemblBacteriaiAAK99560; AAK99560; spr0756.
GeneIDi934298.
KEGGispr:spr0756.
PATRICi19701428. VBIStrPne107296_0836.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007317 Genomic DNA. Translation: AAK99560.1 .
PIRi D97966.
RefSeqi NP_358350.1. NC_003098.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4LP0 X-ray 1.95 A 1-226 [» ]
4LPB X-ray 1.75 A 1-226 [» ]
4MOT X-ray 1.75 A 1-226 [» ]
ProteinModelPortali Q8DQB5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 171101.spr0756.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK99560 ; AAK99560 ; spr0756 .
GeneIDi 934298.
KEGGi spr:spr0756.
PATRICi 19701428. VBIStrPne107296_0836.

Phylogenomic databases

eggNOGi COG0187.
HOGENOMi HOG000075154.
KOi K02622.
OMAi REEFHYE.
OrthoDBi EOG6P334W.

Enzyme and pathway databases

BioCyci SPNE171101:GJC8-763-MONOMER.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPi MF_00939. ParE_type2.
InterProi IPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_ATP-bd.
IPR005740. ParE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsi TIGR01058. parE_Gpos. 1 hit.
PROSITEi PS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-255 / R6.
  2. "Fragment-to-hit-to-lead discovery of a novel pyridylurea scaffold of ATP competitive dual targeting type II topoisomerase inhibiting antibacterial agents."
    Basarab G.S., Manchester J.I., Bist S., Boriack-Sjodin P.A., Dangel B., Illingworth R., Sherer B.A., Sriram S., Uria-Nickelsen M., Eakin A.E.
    J. Med. Chem. 56:8712-8735(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 1-226.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 1-226.

Entry informationi

Entry nameiQ8DQB5_STRR6
AccessioniPrimary (citable) accession number: Q8DQB5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteome

External Data

Dasty 3

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