Reviewed, UniProtKB/Swiss-Prot Q8DPP6 (LACG2_STRR6)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase 2
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase 2
      Short name=PGALase 2
    P-beta-Gal 2
      Short name=PBG 2

Gene names

Name:	lacG2
Ordered Locus Names:	spr1069

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Complete proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	6-phospho-beta-galactosidase 2 HAMAP MF_01574		PRO_0000260733
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8DPP6-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 075F73A048259D7A	FASTA	468	53,927
	10 20 30 40 50 60 MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYNRYPVDL 70 80 90 100 110 120 KLAEEYGVNG IRISIAWSRI FPTGYGQVNA KGVEFYHNLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EALHSNGDFL NRENIEHFVD YAAFCFEEFP EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY 190 200 210 220 230 240 DLAKVFQSHH NMMVSHARAV KLYKDKGYKG EIGVVHALPT KYPLDHENPA DVRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGRYSAETM EGVNHILSVN GGSLDLREED FTALEAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMEAFDGE TEIIHNGKGK KGSSKYQIKG VGRRVAPDYV PRTDWDWIIY PQGLYDQIMR 370 380 390 400 410 420 VKKDYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKQ HLEILSDAIA DGANVKGYFI 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFETQERY PKKSAHWYKK VAETQIID

References

[1]

"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed: 11544234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007317 Genomic DNA. Translation: AAK99872.1.
PIR	D98005.
RefSeq	NP_358662.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
SMR	Q8DPP6. Positions 1-468.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8DPP6.
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Genome annotation databases
GeneID	932827.
GenomeReviews	Gene locus spr1069 in contig AE007317_GR.
KEGG	spr:spr1069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8DPP6.
OMA	TKYPLDP.
Enzyme and pathway databases
BioCyc	SPNE171101:SPR1069-MON.
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG2_STRR6

Accession

Primary (citable) accession number: Q8DPP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)