Q8DNR9 (PHPP_STRR6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase PhpP
EC=3.1.3.16

Alternative name(s):
PP2C-type phosphatase
Ser/Thr phosphoprotein phosphatase
Short name=STPP

Gene names

Name:	phpP
Synonyms:	pppL
Ordered Locus Names:	spr1578

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Reference proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protein phosphatase able to dephosphorylate StkP-P and other phosphorylated protein substrates. PhpP and its cognate protein kinase StkP appear to constitute a functional signaling couple in vivo, PhpP's primary role being probably to control phosphorylation levels of StkP and of its targets. PhpP thus performs an essential control of StkP activity By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm. Note: Mainly localizes to the midcell division sites. Delocalizes from the septum in the presence of antibiotics that target the latest stages of cell-wall biosynthesis and in cells that have stopped dividing. Ref.2
Miscellaneous	Overexpression of PhpP leads to a phenotype comparable to cells lacking stkP, with an increased cell length.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Protein phosphatase PhpP		PRO_0000418148
Sites
	Metal binding	36	1	Manganese 1 By similarity
	Metal binding	36	1	Manganese 2 By similarity
	Metal binding	37	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	192	1	Manganese 2 By similarity
	Metal binding	231	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8DNR9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C7D368C6ECE86E94	FASTA	246	27,103
	10 20 30 40 50 60 MEISLLTDVG QKRTNNQDYV NHYVNRAGRT MIILADGMGG HRAGNIASEM AVTDLGVAWV 70 80 90 100 110 120 DTQIDTVNEV REWFAHYLEI ENQKIHQLGQ DEAYRGMGTT LEVLAIIDNQ AIYAHIGDSR 130 140 150 160 170 180 IGLIRGEEYH QLTSDHSLVN ELLKAGQLTP EEAEAHPQKN IITQSIGQKD EIQPDFGTVI 190 200 210 220 230 240 LESGDYLLLN SDGLTNMISG SEIRDIVTSD IPLADKTETL VRFANNAGGL DNITVALVSM NEEDAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-255 / R6.
[2]	"Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP." Beilharz K., Novakova L., Fadda D., Branny P., Massidda O., Veening J.W. Proc. Natl. Acad. Sci. U.S.A. 109:E905-E913(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, OVEREXPRESSION. Strain: ATCC BAA-255 / R6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007317 Genomic DNA. Translation: AAL00381.1.
PIR	H95201. H98068.
RefSeq	NP_359170.1. NC_003098.1.
3D structure databases
ProteinModelPortal	Q8DNR9.
SMR	Q8DNR9. Positions 1-238.
ModBase	Search...
Protein-protein interaction databases
STRING	171101.spr1578.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL00381; AAL00381; spr1578.
GeneID	933328.
KEGG	spr:spr1578.
PATRIC	19703179. VBIStrPne107296_1705.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000235782.
KO	K01090.
OMA	VSHLGHN.
ProtClustDB	CLSK877236.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHPP_STRR6

Accession

Primary (citable) accession number: Q8DNR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2012
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program