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Q8DL38

- BIOB_THEEB

UniProt

Q8DL38 - BIOB_THEEB

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Protein

Biotin synthase

Gene
bioB, tll0661
Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi101 – 1011Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi170 – 1701Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi230 – 2301Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi300 – 3001Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:tll0661
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus
ProteomesiUP000000440: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Biotin synthaseUniRule annotationPRO_0000381683Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi197221.tll0661.

Structurei

3D structure databases

ProteinModelPortaliQ8DL38.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8DL38-1 [UniParc]FASTAAdd to Basket

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MNSISASAIV LWKNTPLPFV VKSYPMSTLT TDWQHLANLA LADECLSRDQ    50
ARAVLQAPDT ELLNQLAAAY RVRYHYWGNR VRLHYLLNAQ SGLCPEDCHY 100
CSQSKISTAE IEKYPLLSQE KILAAADRAA HLKAGTFCMV ISGRSPSEKV 150
FEQVLAAIRA VKERYPLKIC ACLGLLTPEQ TARLAAAGVD RVNHNLNTSE 200
NFYSEICTTH TFRDRDQTLA NIQAAGITTC SGGILGMGES DDDVIDLALF 250
LRQRQVTSVP VNFLIPIPGT PLGEQHTLNP RHCLRILVLF RFLLPRQEIR 300
IAGGREVHLR SLQPLGLYAA NSMFIGDYLT TPGQAPHQDW QMIADAGFVL 350
EDPEGRAIAP 360
Length:360
Mass (Da):39,878
Last modified:March 1, 2003 - v1
Checksum:iAA370F3D3E6694B1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000039 Genomic DNA. Translation: BAC08212.1.
RefSeqiNP_681450.1. NC_004113.1.
WP_011056508.1. NC_004113.1.

Genome annotation databases

EnsemblBacteriaiBAC08212; BAC08212; BAC08212.
GeneIDi1012044.
KEGGitel:tll0661.
PATRICi23926598. VBITheElo119873_0700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000039 Genomic DNA. Translation: BAC08212.1 .
RefSeqi NP_681450.1. NC_004113.1.
WP_011056508.1. NC_004113.1.

3D structure databases

ProteinModelPortali Q8DL38.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 197221.tll0661.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC08212 ; BAC08212 ; BAC08212 .
GeneIDi 1012044.
KEGGi tel:tll0661.
PATRICi 23926598. VBITheElo119873_0700.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BP-1.

Entry informationi

Entry nameiBIOB_THEEB
AccessioniPrimary (citable) accession number: Q8DL38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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