ID   PDXJ_THEEB              Reviewed;         238 AA.
AC   Q8DKM1;
DT   30-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   10-AUG-2010, entry version 44.
DE   RecName: Full=Pyridoxine 5'-phosphate synthase;
DE            Short=PNP synthase;
DE            EC=2.6.99.2;
GN   Name=pdxJ; OrderedLocusNames=tlr0838;
OS   Thermosynechococcus elongatus (strain BP-1).
OC   Bacteria; Cyanobacteria; Chroococcales; Thermosynechococcus.
OX   NCBI_TaxID=197221;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22225144; PubMed=12240834; DOI=10.1093/dnares/9.4.123;
RA   Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S.,
RA   Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y.,
RA   Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N.,
RA   Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S.;
RT   "Complete genome structure of the thermophilic cyanobacterium
RT   Thermosynechococcus elongatus BP-1.";
RL   DNA Res. 9:123-130(2002).
CC   -!- FUNCTION: Catalyzes the complicated ring closure reaction between
CC       the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and
CC       3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP)
CC       to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-
CC       oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2
CC       H(2)O.
CC   -!- PATHWAY: Cofactor biosynthesis; pyridoxine 5'-phosphate
CC       biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-
CC       phosphate: step 5/5.
CC   -!- SUBUNIT: Homooctamer; tetramer of dimers (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the PNP synthase family.
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DR   EMBL; BA000039; BAC08390.1; -; Genomic_DNA.
DR   RefSeq; NP_681628.1; -.
DR   ProteinModelPortal; Q8DKM1; -.
DR   SMR; Q8DKM1; 4-236.
DR   STRING; Q8DKM1; -.
DR   GeneID; 1011556; -.
DR   GenomeReviews; BA000039_GR; tlr0838.
DR   KEGG; tel:tlr0838; -.
DR   NMPDR; fig|197221.1.peg.837; -.
DR   eggNOG; COG0854; -.
DR   HOGENOM; HBG299314; -.
DR   OMA; AGHGLNY; -.
DR   ProtClustDB; PRK05265; -.
DR   BioCyc; TELO197221:TLR0838-MONOMER; -.
DR   BRENDA; 2.6.99.2; 277225.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033856; F:pyridoxine 5'-phosphate synthase activity; IEA:EC.
DR   GO; GO:0008615; P:pyridoxine biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00279; PdxJ; 1; -.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR004569; PyrdxlP_synth_PdxJ.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF03740; PdxJ; 1.
DR   SUPFAM; SSF63892; PyrdxlP_synth_PdxJ; 1.
DR   TIGRFAMs; TIGR00559; pdxJ; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Pyridoxine biosynthesis; Transferase.
FT   CHAIN         1    238       Pyridoxine 5'-phosphate synthase.
FT                                /FTId=PRO_0000190132.
FT   REGION        9     10       1-deoxy-D-xylulose 5-phosphate binding
FT                                (By similarity).
FT   REGION      213    214       3-amino-2-oxopropyl phosphate binding (By
FT                                similarity).
FT   ACT_SITE     43     43       Proton acceptor (By similarity).
FT   ACT_SITE     70     70       Proton acceptor (By similarity).
FT   ACT_SITE    191    191       Proton donor (By similarity).
FT   BINDING       7      7       3-amino-2-oxopropyl phosphate (By
FT                                similarity).
FT   BINDING      18     18       3-amino-2-oxopropyl phosphate (By
FT                                similarity).
FT   BINDING      45     45       1-deoxy-D-xylulose 5-phosphate (By
FT                                similarity).
FT   BINDING      50     50       1-deoxy-D-xylulose 5-phosphate (By
FT                                similarity).
FT   BINDING     100    100       1-deoxy-D-xylulose 5-phosphate (By
FT                                similarity).
FT   BINDING     192    192       3-amino-2-oxopropyl phosphate; via amide
FT                                nitrogen (By similarity).
FT   SITE        151    151       Transition state stabilizer (By
FT                                similarity).
SQ   SEQUENCE   238 AA;  25658 MW;  6B5C6061C59D65DD CRC64;
     MATLGVNIDH VATIRQARRT VEPDPVAAAL LAELGGADGI TVHLREDRRH IQERDVRLLR
     QTVRTHLNLE MAATPEMVAI ALDIRPDYVT LVPERREEVT TEGGLDVVSQ QEPLTQVVQT
     LQGAGIPVSL FIDADPTQLA AAAKTTAQFI ELHTGRYAEA KGEVAQQREL AILADGVQQA
     KALGLRVNAG HGLTYSNVGA IARLEGIEEL NIGHTIISRA VLVGMVQAVR DMKALISP
//