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Q8DKF1 (GSHB_THEEB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:tlr0908
OrganismThermosynechococcus elongatus (strain BP-1) [Reference proteome] [HAMAP]
Taxonomic identifier197221 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197487

Regions

Domain133 – 317185ATP-grasp
Nucleotide binding159 – 21557ATP By similarity

Sites

Metal binding2881Magnesium or manganese By similarity
Metal binding2901Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8DKF1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 39B48E395E173873

FASTA31835,063
        10         20         30         40         50         60 
MDIAFIIDPI ASLDPGHDTS VALMEAAQAA GARVWVTEIS QLLIREGQVW AALTPIQLSP 

        70         80         90        100        110        120 
VQLVDGQWQI PQPWFELGAL EWRPLNTFRA VWMRKDPPVN TAYLYATYCL DLVDPQTTLV 

       130        140        150        160        170        180 
LNSPAGLRHA NEKMYALQFT SVIPKTIVTA DKQRIREFVQ QQGMAVLKPL GGKGGEGILF 

       190        200        210        220        230        240 
LQAGDRNLNS MIEISTQRGQ LPVMLQEYLP AAKEGDKRII LLNGEPIGAV NRIPTGDEFR 

       250        260        270        280        290        300 
GNMATGGRVA AAEITERDRQ ICQTLAPALR RDGLYFVGID VIGGYLTEVN VTSPTGVREI 

       310 
DRLNGTRLGQ QVMAWLFS 

« Hide

References

[1]"Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1."
Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S., Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takeuchi C., Yamada M., Tabata S.
DNA Res. 9:123-130(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BP-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000039 Genomic DNA. Translation: BAC08460.1.
RefSeqNP_681698.1. NC_004113.1.

3D structure databases

ProteinModelPortalQ8DKF1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING197221.tlr0908.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC08460; BAC08460; BAC08460.
GeneID1011416.
KEGGtel:tlr0908.
PATRIC23927108. VBITheElo119873_0954.

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAEISTKQG.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_THEEB
AccessionPrimary (citable) accession number: Q8DKF1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways