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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (tlr1234), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi349Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi409Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi412Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciTELO197221:G1G3I-927-MONOMER
UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:tlr0909
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeThermosynechococcus
Proteomesi
  • UP000000440 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000768281 – 4693-isopropylmalate dehydratase large subunitAdd BLAST469

Proteomic databases

PRIDEiQ8DKF0

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi197221.tlr0909

Structurei

3D structure databases

ProteinModelPortaliQ8DKF0
SMRiQ8DKF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQI Bacteria
COG0065 LUCA
HOGENOMiHOG000226972
KOiK01703
OMAiFDHQVPA
OrthoDBiPOG091H02GD

Family and domain databases

CDDicd01583 IPMI, 1 hit
Gene3Di3.30.499.10, 1 hit
HAMAPiMF_01026 LeuC_type1, 1 hit
InterProiView protein in InterPro
IPR004430 3-IsopropMal_deHydase_lsu
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR033941 IPMI_cat
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR00170 leuC, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8DKF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMSRGTLFD KVWEQHTVAT LPSGQTQLFI GLHLIHEVTS PQAFAMLRER
60 70 80 90 100
GLPVLYPQRT IATVDHIVPT DTLARPLQDA LAEEMLQALE ANCRAHNIPF
110 120 130 140 150
FGIGSGRQGI VHVIAPEQGL TQPGMTIACG DSHTSTHGAF GAIAFGIGTS
160 170 180 190 200
QVRDVLATQT LALNKLKVRK VEVNGSLAAG VYAKDVILHV IRHLGVKGGV
210 220 230 240 250
GYAYEFAGTT FGQLSMEERM TVCNMAIEGG ARCGYVNPDE TTFAYLKGRP
260 270 280 290 300
FAPQGKAWDE AVAWWRSLAS DADAEYDDVV TFAAADIAPT VTWGITPGQS
310 320 330 340 350
VAVDETLPTL ESLPVAERAI AAEAYAYMDL QPGQPIQGTK IDVCFIGSCT
360 370 380 390 400
NGRISDLRQA AKVVEGRKVK PGVKAFVVPG SERVKAQAEA EGLDVVFKAA
410 420 430 440 450
GFEWRNPGCS MCLAMNPDKL QGRQISASSS NRNFKGRQGS PSGRTLLMSP
460
AMVAAAAIAG EVTDVRAFL
Length:469
Mass (Da):50,088
Last modified:March 1, 2003 - v1
Checksum:iD3127080A899B236
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA Translation: BAC08461.1
RefSeqiNP_681699.1, NC_004113.1
WP_011056753.1, NC_004113.1

Genome annotation databases

EnsemblBacteriaiBAC08461; BAC08461; BAC08461
GeneIDi1011413
KEGGitel:tlr0909
PATRICifig|197221.4.peg.955

Similar proteinsi

Entry informationi

Entry nameiLEUC_THEEB
AccessioniPrimary (citable) accession number: Q8DKF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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