Q8DK29 (ISPH_THEEB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	tlr1041

Organism

Thermosynechococcus elongatus (strain BP-1)

Taxonomic identifier

197221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Thermosynechococcus

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128877

Sequences

	Sequence		Length	Mass (Da)
	Q8DK29 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: FB0851B618B73510	FASTA	402	45,213
	10 20 30 40 50 60 MDTRAFKRSL HSSENYHRKG FGHGEEVNQQ LQGEYQSSLI QQIRANGYRW QQGDVTIRLA 70 80 90 100 110 120 EAFGFCWGVE RAVALAYETR THFPTERIWI TNEIIHNPSV NERLRQMAVE FIPVVNGVKD 130 140 150 160 170 180 FSGVRPGDVV ILPAFGASVQ EMQLLNERGC TIVDTTCPWV SKVWHSVEKH KKVSFTSIIH 190 200 210 220 230 240 GKYNHEETIA TSSFAGTYLI VLNLEEARYV CDYILHGGDR AAFMAKFAKA CSPGFDPDRD 250 260 270 280 290 300 LVRVGIANQT TMLKGETEQI GKLFERTMIQ KYGPDRLNEH FMSFNTICDA TQERQDAMLS 310 320 330 340 350 360 LVKEPLDLMV VIGGYNSSNT THLQEIAIEH GIPSYHIDSA DRIGPGNRIE HKPLHQNPTV 370 380 390 400 AENWLPDRPI TIGITSGAST PDKVVEEVLN KIFALRSVAT VS

References

[1]

"Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1." Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S., Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M. , Takeuchi C., Yamada M., Tabata S. DNA Res. 9:123-130(2002) [PubMed: 12240834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BP-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000039 Genomic DNA. Translation: BAC08594.1.
RefSeq	NP_681832.1. NC_004113.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8DK29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1012362.
GenomeReviews	Gene locus tlr1041 in contig BA000039_GR.
KEGG	tel:tlr1041.
NMPDR	fig|197221.1.peg.1041.
PATRIC	23927386. VBITheElo119873_1093.
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG286221.
OMA	VQEMQLL.
ProtClustDB	PRK13371.
Enzyme and pathway databases
BioCyc	TELO197221:TLR1041-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_THEEB

Accession

Primary (citable) accession number: Q8DK29

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program