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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[3Fe-4S] clusterUniRule annotationNote: Binds 1 [3Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei96 – 961SubstrateUniRule annotation
Binding sitei135 – 1351SubstrateUniRule annotation
Metal bindingi157 – 1571Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotation
Binding sitei250 – 2501SubstrateUniRule annotation
Metal bindingi288 – 2881Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei379 – 3791SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Gene namesi
Name:ispHUniRule annotation
Ordered Locus Names:tlr1041
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus
Proteomesi
  • UP000000440 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4024024-hydroxy-3-methylbut-2-enyl diphosphate reductasePRO_0000128877Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi197221.tlr1041.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni317 – 3193Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RAJ. Bacteria.
COG0761. LUCA.
HOGENOMiHOG000240388.
KOiK03527.
OMAiAYETRRH.
OrthoDBiEOG6HF624.

Family and domain databases

HAMAPiMF_00191. IspH.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8DK29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTRAFKRSL HSSENYHRKG FGHGEEVNQQ LQGEYQSSLI QQIRANGYRW
60 70 80 90 100
QQGDVTIRLA EAFGFCWGVE RAVALAYETR THFPTERIWI TNEIIHNPSV
110 120 130 140 150
NERLRQMAVE FIPVVNGVKD FSGVRPGDVV ILPAFGASVQ EMQLLNERGC
160 170 180 190 200
TIVDTTCPWV SKVWHSVEKH KKVSFTSIIH GKYNHEETIA TSSFAGTYLI
210 220 230 240 250
VLNLEEARYV CDYILHGGDR AAFMAKFAKA CSPGFDPDRD LVRVGIANQT
260 270 280 290 300
TMLKGETEQI GKLFERTMIQ KYGPDRLNEH FMSFNTICDA TQERQDAMLS
310 320 330 340 350
LVKEPLDLMV VIGGYNSSNT THLQEIAIEH GIPSYHIDSA DRIGPGNRIE
360 370 380 390 400
HKPLHQNPTV AENWLPDRPI TIGITSGAST PDKVVEEVLN KIFALRSVAT

VS
Length:402
Mass (Da):45,213
Last modified:March 1, 2003 - v1
Checksum:iFB0851B618B73510
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA. Translation: BAC08594.1.
RefSeqiNP_681832.1. NC_004113.1.
WP_011056884.1. NC_004113.1.

Genome annotation databases

EnsemblBacteriaiBAC08594; BAC08594; BAC08594.
GeneIDi1012362.
KEGGitel:tlr1041.
PATRICi23927386. VBITheElo119873_1093.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA. Translation: BAC08594.1.
RefSeqiNP_681832.1. NC_004113.1.
WP_011056884.1. NC_004113.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi197221.tlr1041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC08594; BAC08594; BAC08594.
GeneIDi1012362.
KEGGitel:tlr1041.
PATRICi23927386. VBITheElo119873_1093.

Phylogenomic databases

eggNOGiENOG4107RAJ. Bacteria.
COG0761. LUCA.
HOGENOMiHOG000240388.
KOiK03527.
OMAiAYETRRH.
OrthoDBiEOG6HF624.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Family and domain databases

HAMAPiMF_00191. IspH.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BP-1.

Entry informationi

Entry nameiISPH_THEEB
AccessioniPrimary (citable) accession number: Q8DK29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.