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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi66Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei96SubstrateUniRule annotation1
Metal bindingi157Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei185SubstrateUniRule annotation1
Active sitei187Proton donorUniRule annotation1
Binding sitei250SubstrateUniRule annotation1
Metal bindingi288Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei379SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Short name:
HMBPP reductaseUniRule annotation
Gene namesi
Name:ispHUniRule annotation
Ordered Locus Names:tlr1041
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeThermosynechococcus
Proteomesi
  • UP000000440 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001288771 – 4024-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST402

Interactioni

Protein-protein interaction databases

STRINGi197221.tlr1041.

Structurei

3D structure databases

SMRiQ8DK29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni317 – 319Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RAJ. Bacteria.
COG0761. LUCA.
HOGENOMiHOG000240388.
KOiK03527.
OMAiTKVHKEA.
OrthoDBiPOG091H038O.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiView protein in InterPro
IPR003451. LytB/IspH.
PfamiView protein in Pfam
PF02401. LYTB. 1 hit.
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8DK29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTRAFKRSL HSSENYHRKG FGHGEEVNQQ LQGEYQSSLI QQIRANGYRW
60 70 80 90 100
QQGDVTIRLA EAFGFCWGVE RAVALAYETR THFPTERIWI TNEIIHNPSV
110 120 130 140 150
NERLRQMAVE FIPVVNGVKD FSGVRPGDVV ILPAFGASVQ EMQLLNERGC
160 170 180 190 200
TIVDTTCPWV SKVWHSVEKH KKVSFTSIIH GKYNHEETIA TSSFAGTYLI
210 220 230 240 250
VLNLEEARYV CDYILHGGDR AAFMAKFAKA CSPGFDPDRD LVRVGIANQT
260 270 280 290 300
TMLKGETEQI GKLFERTMIQ KYGPDRLNEH FMSFNTICDA TQERQDAMLS
310 320 330 340 350
LVKEPLDLMV VIGGYNSSNT THLQEIAIEH GIPSYHIDSA DRIGPGNRIE
360 370 380 390 400
HKPLHQNPTV AENWLPDRPI TIGITSGAST PDKVVEEVLN KIFALRSVAT

VS
Length:402
Mass (Da):45,213
Last modified:March 1, 2003 - v1
Checksum:iFB0851B618B73510
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA. Translation: BAC08594.1.
RefSeqiNP_681832.1. NC_004113.1.
WP_011056884.1. NC_004113.1.

Genome annotation databases

EnsemblBacteriaiBAC08594; BAC08594; BAC08594.
GeneIDi1012362.
KEGGitel:tlr1041.
PATRICifig|197221.4.peg.1093.

Similar proteinsi

Entry informationi

Entry nameiISPH_THEEB
AccessioniPrimary (citable) accession number: Q8DK29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2003
Last modified: October 25, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families