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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi109 – 1091Magnesium; catalyticUniRule annotation
Sitei110 – 1101Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei134 – 1341Proton acceptorUniRule annotation
Binding sitei169 – 1691Substrate; shared with dimeric partnerUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Binding sitei266 – 2661Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 822ATPUniRule annotation
Nucleotide bindingi108 – 1114ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:tll1316
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus
ProteomesiUP000000440: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369ATP-dependent 6-phosphofructokinasePRO_0000111997Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi197221.tll1316.

Structurei

3D structure databases

ProteinModelPortaliQ8DJB1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1343Substrate bindingUniRule annotation
Regioni176 – 1783Substrate bindingUniRule annotation
Regioni272 – 2754Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiMVLHNQE.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DJB1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSRKRLGV FTSGGDCPGL NTAIRAIVAH ATLSYGWEVL GILHATQGLI
60 70 80 90 100
ERKAIPLNAE GLGGMDVLLN MGGTILGAIN KGDTLGHADE VIAGYYELGL
110 120 130 140 150
DALIAICGDG SLRILHQLAQ KGNWNFLAIP KTIDNDVALT DRAIGFDTAV
160 170 180 190 200
NTVVEALNRI TSTAASHDRV FVVEVMGRTA GHLALYSGIA GGADIILIPE
210 220 230 240 250
IPYSIEGICQ HLQKLRDRWG RQFALIVVAE GSKQLEEDAN HPRHCSIGQY
260 270 280 290 300
IADKIAQHSP IPIELRVSVL GHIQRGGAPM AMDRLLAAGM GNTAVDLAAQ
310 320 330 340 350
GTFGRMVAWQ AGQVVTVPIA DVVAKCPRHV DPNSFLIRTA QGLGIYVGDK
360
PMLPYVDPTL CRDQVICAI
Length:369
Mass (Da):39,271
Last modified:March 1, 2003 - v1
Checksum:i2FE4A8C249105916
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA. Translation: BAC08868.1.
RefSeqiNP_682106.1. NC_004113.1.

Genome annotation databases

EnsemblBacteriaiBAC08868; BAC08868; BAC08868.
GeneIDi1012416.
KEGGitel:tll1316.
PATRICi23927976. VBITheElo119873_1384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA. Translation: BAC08868.1.
RefSeqiNP_682106.1. NC_004113.1.

3D structure databases

ProteinModelPortaliQ8DJB1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi197221.tll1316.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC08868; BAC08868; BAC08868.
GeneIDi1012416.
KEGGitel:tll1316.
PATRICi23927976. VBITheElo119873_1384.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiMVLHNQE.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BP-1.

Entry informationi

Entry nameiPFKA_THEEB
AccessioniPrimary (citable) accession number: Q8DJB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: February 4, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.