Q8DIF1 (GLO2_THEEB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	tll1639

Organism

Thermosynechococcus elongatus (strain BP-1)

Taxonomic identifier

197221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Thermosynechococcus

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000068224
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 1 By similarity
	Metal binding	132	1	Zinc 1 By similarity
	Metal binding	132	1	Zinc 2 By similarity
	Metal binding	170	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8DIF1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 48913B3B70A0DF39	FASTA	252	28,032
	10 20 30 40 50 60 MMIYRLNALT DNYIFLLHDP QTGTAAVVDP AEPEPVLAKL AELGATLRAI FNTHHHWDHV 70 80 90 100 110 120 GANCALRSRF PDIAVYGSSE DQGRIPEQTV FLKAGDRVPF GQTYFDVLFV PGHTRGHIAY 130 140 150 160 170 180 YAPTTGDLFC GDTLFGGGCG RLFEGSPAQM LDSLNQLRQL PEETRVWCAH EYTQKNLSFA 190 200 210 220 230 240 LTVEADNPTL QERYAQVCRD RAQGKATIPS TIGLEKATNP FLRCEVRSIQ IAVGATTPLQ 250 TFTRLRGKRD QY

References

[1]

"Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1." Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S., Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M. , Takeuchi C., Yamada M., Tabata S. DNA Res. 9:123-130(2002) [PubMed: 12240834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BP-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000039 Genomic DNA. Translation: BAC09191.1.
RefSeq	NP_682429.1. NC_004113.1.
3D structure databases
HSSP	HSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
ProteinModelPortal	Q8DIF1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8DIF1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1012092.
GenomeReviews	Gene locus tll1639 in contig BA000039_GR.
KEGG	tel:tll1639.
NMPDR	fig|197221.1.peg.1638.
PATRIC	23928652. VBITheElo119873_1719.
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	IGCGRVF.
Enzyme and pathway databases
BioCyc	TELO197221:TLL1639-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_THEEB

Accession

Primary (citable) accession number: Q8DIF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program