Reviewed, UniProtKB/Swiss-Prot Q8DI68 (ACSF2_THEEB)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase 2
    EC=1.14.13.81

Gene names

Name:	acsF2
Ordered Locus Names:	tlr1722

Organism

Thermosynechococcus elongatus (strain BP-1) [Complete proteome] [HAMAP]

Taxonomic identifier

197221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Thermosynechococcus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity. HAMAP MF_01840
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity. HAMAP MF_01840
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent). HAMAP MF_01840
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2 HAMAP MF_01840		PRO_0000217536

Sequences

	Sequence		Length	Mass (Da)
	Q8DI68-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F341D573D305BCC2	FASTA	354	41,683
	10 20 30 40 50 60 MVAIAPNPHS GQSVKTIREN LLTPRFYTTD FEQAAKLDLS RQQEQLEAML AEMRADYNRH 70 80 90 100 110 120 HFVRDDSFKG TWDLLDAQTR KAFIDYLERS CVSEFSGFLL FKELSRKLKD RNPLLAEIFH 130 140 150 160 170 180 LMARDEARHA GFLNKAMMDF GHAMDLGYLS KTRTYTFFPL PWVLYTVYLS EKIGYWRYII 190 200 210 220 230 240 IYRHLEKHPE HSFYPLFNYF ERWCQDENRH GDIFKALIHS QPQLIQGWGA KLWMRFFLLT 250 260 270 280 290 300 VFATHTLTVH ERAKFYEALG LDATAFDREV IAKTNETAAR TFSVVLNVDH PEFYSRLQRC 310 320 330 340 350 VEISNKLQAI EATHQPKWLK ALRKLPHQLA IAGHLLRIYL LPPVNAQQEW GTVH

References

[1]

"Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1." Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S., Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M. , Takeuchi C., Yamada M., Tabata S. DNA Res. 9:123-130(2002) [PubMed: 12240834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000039 Genomic DNA. Translation: BAC09274.1.
RefSeq	NP_682512.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8DI68.
Genome annotation databases
GeneID	1010584.
GenomeReviews	Gene locus tlr1722 in contig BA000039_GR.
KEGG	tel:tlr1722.
NMPDR	fig|197221.1.peg.1721.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG76047.
HOGENOM	HBG284843.
OMA	NRHGDIF.
Enzyme and pathway databases
BioCyc	TELO197221:TLR1722-MONOMER.
BRENDA	1.14.13.81. 277225.
Family and domain databases
HAMAP	MF_01840. AcsF. [Tree]
InterPro	IPR008434. AcsF. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF2_THEEB

Accession

Primary (citable) accession number: Q8DI68

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)