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Q8DGI7

- SYI_THEEB

UniProt

Q8DGI7 - SYI_THEEB

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
    Binding sitei608 – 6081ATPUniRule annotation
    Metal bindingi922 – 9221ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation
    Metal bindingi942 – 9421ZincUniRule annotation
    Metal bindingi945 – 9451ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:tlr2330
    OrganismiThermosynechococcus elongatus (strain BP-1)
    Taxonomic identifieri197221 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus
    ProteomesiUP000000440: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Isoleucine--tRNA ligasePRO_0000098489Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi197221.tlr2330.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8DGI7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 7111"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8DGI7-1 [UniParc]FASTAAdd to Basket

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    MTDATPDYKD TVNLPQTTFE MRANAATREP QLQAFWAQHK IYETLQQTNP    50
    GEVFILHDGP PYANGALHIG HALNKILKDI INKYQLLRGR KVHYRPGWDC 100
    HGLPIELKVL QNLKPEQRAQ LTPLTLRQQA KEFALKTVAE QKQSFQRYGV 150
    WGDWAHPYLT LTPDYEAAQI GVFGEMVLRG YIYRGLKPVH WSPSSKTALA 200
    EAELEYPEGH TSRSLYAAFE VIELPQGLAK AWYNALGKLG VAIWTTTPWT 250
    IPANLAVSVN PDLTYALVEV QPSERYKHLI VAKDLVERLS DTLGRTLKVV 300
    ATAKGADLEH SRYRHPLFER EGKILIGGDY VTTESGTGLV HTAPGHGLED 350
    YGVGQRYGLP ILSPVDENGC FTAEAGPFAG LNVLQEGNAA VIAALQECGA 400
    LLKEEPYVHK YPYDWRTKKP TIFRATEQWF ASVEGFRDAA LRAIAEVKWI 450
    PAQGENRITA MVAERSDWCI SRQRSWGVPI PVFYDKETGE PLLTAETIAH 500
    VQAIIRDRGS DAWWELSVAE LLPESLRDQA DRYEKGTDTM DVWFDSGSSW 550
    AAVLGDQQAD LYLEGSDQHR GWFQSSLLTR VAVKGQAPYK AVLTHGFVLD 600
    EQGRKMSKSL GNVTDPREVI EGGKNQKQDP PYGADVLRLW VSSVDYANDV 650
    PIGKNILRQL ADVYRKIRNT ARFLLGNLHD FEPEQDMIPY AQLPALDRYM 700
    LHRLHEVFSE VTAAFDSFQF YRFFQTIQNL CVVDLSNFYL DIAKDRLYIS 750
    AATGDRRRSC QTVLAIALQN LARAIAPVLP HLAEDIWQHL PFKTPYLSVF 800
    QSGWVQLSAQ WQDNALAAEW QRLRQLRLEV NKVLEKARAE KLIGSSLEAK 850
    VWLYVADSEW RDRLAQMNPR DALSGNGVDE LRYLFLVSQV ELMPQPQAVD 900
    VPYVLEAEGL WIGVGHAQGE KCVRCWNYSE SVGQSALHPQ LCDRCQAALQ 950
    GEF 953
    Length:953
    Mass (Da):107,523
    Last modified:December 20, 2005 - v2
    Checksum:iEDC235E0D3C77843
    GO

    Sequence cautioni

    The sequence BAC09882.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000039 Genomic DNA. Translation: BAC09882.1. Different initiation.
    RefSeqiNP_683120.1. NC_004113.1.

    Genome annotation databases

    EnsemblBacteriaiBAC09882; BAC09882; BAC09882.
    GeneIDi1011751.
    KEGGitel:tlr2330.
    PATRICi23930126. VBITheElo119873_2442.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000039 Genomic DNA. Translation: BAC09882.1 . Different initiation.
    RefSeqi NP_683120.1. NC_004113.1.

    3D structure databases

    ProteinModelPortali Q8DGI7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 197221.tlr2330.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC09882 ; BAC09882 ; BAC09882 .
    GeneIDi 1011751.
    KEGGi tel:tlr2330.
    PATRICi 23930126. VBITheElo119873_2442.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BP-1.

    Entry informationi

    Entry nameiSYI_THEEB
    AccessioniPrimary (citable) accession number: Q8DGI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3