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Q8DGI7

- SYI_THEEB

UniProt

Q8DGI7 - SYI_THEEB

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi942 – 9421ZincUniRule annotation
Metal bindingi945 – 9451ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:tlr2330
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus
ProteomesiUP000000440: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligasePRO_0000098489Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi197221.tlr2330.

Structurei

3D structure databases

ProteinModelPortaliQ8DGI7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DGI7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDATPDYKD TVNLPQTTFE MRANAATREP QLQAFWAQHK IYETLQQTNP
60 70 80 90 100
GEVFILHDGP PYANGALHIG HALNKILKDI INKYQLLRGR KVHYRPGWDC
110 120 130 140 150
HGLPIELKVL QNLKPEQRAQ LTPLTLRQQA KEFALKTVAE QKQSFQRYGV
160 170 180 190 200
WGDWAHPYLT LTPDYEAAQI GVFGEMVLRG YIYRGLKPVH WSPSSKTALA
210 220 230 240 250
EAELEYPEGH TSRSLYAAFE VIELPQGLAK AWYNALGKLG VAIWTTTPWT
260 270 280 290 300
IPANLAVSVN PDLTYALVEV QPSERYKHLI VAKDLVERLS DTLGRTLKVV
310 320 330 340 350
ATAKGADLEH SRYRHPLFER EGKILIGGDY VTTESGTGLV HTAPGHGLED
360 370 380 390 400
YGVGQRYGLP ILSPVDENGC FTAEAGPFAG LNVLQEGNAA VIAALQECGA
410 420 430 440 450
LLKEEPYVHK YPYDWRTKKP TIFRATEQWF ASVEGFRDAA LRAIAEVKWI
460 470 480 490 500
PAQGENRITA MVAERSDWCI SRQRSWGVPI PVFYDKETGE PLLTAETIAH
510 520 530 540 550
VQAIIRDRGS DAWWELSVAE LLPESLRDQA DRYEKGTDTM DVWFDSGSSW
560 570 580 590 600
AAVLGDQQAD LYLEGSDQHR GWFQSSLLTR VAVKGQAPYK AVLTHGFVLD
610 620 630 640 650
EQGRKMSKSL GNVTDPREVI EGGKNQKQDP PYGADVLRLW VSSVDYANDV
660 670 680 690 700
PIGKNILRQL ADVYRKIRNT ARFLLGNLHD FEPEQDMIPY AQLPALDRYM
710 720 730 740 750
LHRLHEVFSE VTAAFDSFQF YRFFQTIQNL CVVDLSNFYL DIAKDRLYIS
760 770 780 790 800
AATGDRRRSC QTVLAIALQN LARAIAPVLP HLAEDIWQHL PFKTPYLSVF
810 820 830 840 850
QSGWVQLSAQ WQDNALAAEW QRLRQLRLEV NKVLEKARAE KLIGSSLEAK
860 870 880 890 900
VWLYVADSEW RDRLAQMNPR DALSGNGVDE LRYLFLVSQV ELMPQPQAVD
910 920 930 940 950
VPYVLEAEGL WIGVGHAQGE KCVRCWNYSE SVGQSALHPQ LCDRCQAALQ

GEF
Length:953
Mass (Da):107,523
Last modified:December 20, 2005 - v2
Checksum:iEDC235E0D3C77843
GO

Sequence cautioni

The sequence BAC09882.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000039 Genomic DNA. Translation: BAC09882.1. Different initiation.
RefSeqiNP_683120.1. NC_004113.1.

Genome annotation databases

EnsemblBacteriaiBAC09882; BAC09882; BAC09882.
GeneIDi1011751.
KEGGitel:tlr2330.
PATRICi23930126. VBITheElo119873_2442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000039 Genomic DNA. Translation: BAC09882.1 . Different initiation.
RefSeqi NP_683120.1. NC_004113.1.

3D structure databases

ProteinModelPortali Q8DGI7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 197221.tlr2330.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC09882 ; BAC09882 ; BAC09882 .
GeneIDi 1011751.
KEGGi tel:tlr2330.
PATRICi 23930126. VBITheElo119873_2442.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BP-1.

Entry informationi

Entry nameiSYI_THEEB
AccessioniPrimary (citable) accession number: Q8DGI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3