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Reviewed, UniProtKB/Swiss-Prot Q8DFG4 (5NTD_VIBVU)

Last modified October 13, 2009. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    5'-nucleotidase
    EC=3.1.3.5
Gene names
Name: nutA
Ordered Locus Names: VV1_0248
OrganismVibrio vulnificus [Complete proteome] [HAMAP]
Taxonomic identifier672 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

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Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Degradation of extracellular 5'-nucleotides for nutritional needs By similarity.

Catalytic activity

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactor

Chloride By similarity.

Magnesium By similarity.

Subcellular location

Cell outer membrane; Lipid-anchor Probable.

Sequence similarities

Belongs to the 5'-nucleotidase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 5535325'-nucleotidase
PRO_0000000030

Regions

Region501 – 5077Substrate binding By similarity

Sites

Metal binding451Divalent metal cation 1 By similarity
Metal binding471Divalent metal cation 1 By similarity
Metal binding881Divalent metal cation 1 By similarity
Metal binding881Divalent metal cation 2 By similarity
Metal binding1201Divalent metal cation 2 By similarity
Metal binding2211Divalent metal cation 2 By similarity
Metal binding2561Divalent metal cation 2 By similarity
Metal binding2581Divalent metal cation 1 By similarity
Binding site4321Substrate By similarity
Site1211Transition state stabilizer By similarity
Site1241Transition state stabilizer By similarity

Amino acid modifications

Lipidation221N-palmitoyl cysteine Probable
Lipidation221S-diacylglycerol cysteine Probable

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Sequences

Sequence LengthMass (Da)Tools
Q8DFG4-1 [UniParc].

Last modified November 21, 2003. Version 2.
Checksum: A4A63360A44C27A6

FASTA55361,331
        10         20         30         40         50         60 
MKQRLIVKTA LSAAILATLA GCATQPTQEW AADTTYKLTV LHTNDHHGRF WQNKYGEYGM 

        70         80         90        100        110        120 
AARKTLIDEL RAEIQAEGGS VLLLSGGDIN TGVPESDLQD AEPDFKGMSK IGYDAMALGN 

       130        140        150        160        170        180 
HEFDNPLDVL MKQKEWANFP MLSANIYDKK TGERMFQAYE MFDKQGIKIA VIGLTTEDTA 

       190        200        210        220        230        240 
KLGNPEFIGA IDFRDPKEEA KKLIAELKET EKPDLIFAVT HMGHYEDGKR GINAPGDVAL 

       250        260        270        280        290        300 
ARYLNEGDLD MIVGGHSQEP VCMEAPNVVK KNFKPADECK PDQQNGTYIV QAHEWGKYVG 

       310        320        330        340        350        360 
RADYEFRNGE LRMVSYDLIP VNLKKKVEVD GKSQRVFIES EIKEDTALLE FLRPYQEKGQ 

       370        380        390        400        410        420 
EQLNVKIADT NGKLEGDRNV VRFQQTNLGR LIAVSHMERA KADFAVMNSG GVRDSIEAGE 

       430        440        450        460        470        480 
VTYKDVLTVQ PFANILTYTD MTGKEVLDYL NVVATKPVDS GAYAQFAGIS MTVANGKVSN 

       490        500        510        520        530        540 
VFIGGKQLRL DETYRFTVPS YNAAGGDGYP KLTGHPGYVN TGFVDAEVLK EFLEKNSPID 

       550 
VNKFAPNGEI VYK

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References

[1]"Complete genome sequence of Vibrio vulnificus CMCP6."
Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CMCP6.

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Cross-references

Sequence databases

AE016795 Genomic DNA. Translation: AAO08784.1. Different initiation.
RefSeqNP_759257.1.

3D structure databases

HSSPHSSP built from PDB template 2USH based on UniProtKB P07024.
ModBaseSearch...

Genome annotation databases

GeneID1177201.
GenomeReviewsGene locus VV1_0248 in contig AE016795_GR.
KEGGvvu:VV1_0248.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ8DFG4.

Enzyme and pathway databases

BioCycVVUL216895:VV1_0248-MON.
BRENDA3.1.3.5. 277169.

Family and domain databases

InterProIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. M-pesterase.
[Graphical view]
Gene3DG3DSA:3.90.780.10. 5'-Nucleotdase_C. 1 hit.
PANTHERPTHR11575. 5_nucleotidase. 1 hit.
PfamPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR01607. APYRASEFAMLY.
PROSITEPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry name5NTD_VIBVU
AccessionPrimary (citable) accession number: Q8DFG4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: November 21, 2003
Last modified: October 13, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents