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Q8DF81 (PUR4_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:VV1_0340
OrganismVibrio vulnificus [Complete proteome] [HAMAP]
Taxonomic identifier672 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length1297 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12971297Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100422

Regions

Domain1044 – 1297254Glutamine amidotransferase type-1
Nucleotide binding307 – 31812ATP Potential

Sites

Active site11371Nucleophile By similarity
Active site12621 By similarity
Active site12641 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8DF81 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: C8FBA0BC243CEF32

FASTA1,297140,847
        10         20         30         40         50         60 
MRILRGSPAL SEFRVNKLLE LCREQDLPVT GIYAEFMHFA DLTSELDAEA LEKLEKLLTY 

        70         80         90        100        110        120 
GPTIEEHEPQ GLLLLVTPRP GTISPWSSKA TDIAQNCGLN AVKRLERGTA YYVESSSELS 

       130        140        150        160        170        180 
SVQIDIVKSI IHDRMMEAVF GDLEAAAALF SVAQPAPMTQ VDILSGGRLA LEEANVSLGL 

       190        200        210        220        230        240 
ALAEDEIDYL VENFTKLGRN PNDIELMMFA QANSEHCRHK IFNADWTIDG VEQPKSLFKM 

       250        260        270        280        290        300 
IKNTFETTPD HVLSAYKDNA AVMTGSKVGR FFPDPETRQY NYHHEDAHIL MKVETHNHPT 

       310        320        330        340        350        360 
AISPWPGAST GSGGEIRDEG ATGIGGKPKA GLVGFTTSNL RIPGFEQPWE TDFGKPGRIV 

       370        380        390        400        410        420 
NALDIMLEGP LGGAAFNNEF GRPNLLGYFR TYEEKVTSHA GEEVRGYHKP IMIAGGMGNI 

       430        440        450        460        470        480 
RDEHVQKKEI PVGASLIVLG GPAMNIGLGG GAASSMASGQ SAEDLDFASV QRENPEMERR 

       490        500        510        520        530        540 
CQEVIDRCWQ LGDNNPIAFI HDVGAGGISN ALPELVNDGE RGGKFQLRDV PNDEPGMSPL 

       550        560        570        580        590        600 
EIWCNESQER YVLAVAPENM AAFDAICKRE RAPYAVVGVA TEERHLTLED AHFDNTPIDM 

       610        620        630        640        650        660 
PMDILLGKPP KMHREATTLK VDSPAMTRDG IELNEAVDRV LRLPTVAEKT FLITIGDRTV 

       670        680        690        700        710        720 
TGLVARDQMV GPWQVPVANC AVTAASYDTY HGEAMSMGER TPVALLDFGA SARLAVGESL 

       730        740        750        760        770        780 
TNIAATDIGD IKRIKLSANW MSPAGHPGED AGLYEAVKAV GEELCPALGL TIPVGKDSMS 

       790        800        810        820        830        840 
MKTKWNENGE EKEVTSPLSL IITAFARVED VRKTITPQLR TDKGETSLVL VDLGNGKNRL 

       850        860        870        880        890        900 
GATALAQVYK QLGDKPADVD NAEQLKGFFD AMQALVRQDK LLAYHDKGDG GLLVTLAEMA 

       910        920        930        940        950        960 
FAGHCGVNAN IAALGDDVLA ALFNEELGAV VQVKNDELDS VLSTLAANGL EACSHVIGAI 

       970        980        990       1000       1010       1020 
DASDNFVIRS GDAVILERSR TDLRVIWAET THKMQALRDN PACADQEFEA KKDNSDPGLN 

      1030       1040       1050       1060       1070       1080 
VSLSFDVNED IAAPYIAKGA KPKMAILREQ GVNSHVEMAA AFDRAGFEAT DIHMSDILTG 

      1090       1100       1110       1120       1130       1140 
QAVLDEYHGL VACGGFSYGD VLGAGEGWAK SVLFNAQARE QFQAFFNREN TFSLGVCNGC 

      1150       1160       1170       1180       1190       1200 
QMLSNLKELI PGADLWPRFV RNESERFEAR FSLVEVQKSD SVFFDGMAGS RMPIAVSHGE 

      1210       1220       1230       1240       1250       1260 
GRVEVRDAQH LAAIEASGTV AVRFVDNLGN PTQQYPNNPN GSPNAITGLT TKDGRVTIMM 

      1270       1280       1290 
PHPERVFRTV ANSWAPEGWG ENGAWMRMFQ NARKNLG

« Hide

References

[1]"Complete genome sequence of Vibrio vulnificus CMCP6."
Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CMCP6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016795 Genomic DNA. Translation: AAO08867.1.
RefSeqNP_759340.1. NC_004459.3.

3D structure databases

ProteinModelPortalQ8DF81.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1177299.
GenomeReviewsGene locus VV1_0340 in contig AE016795_GR.
KEGGvvu:VV1_0340.
PATRIC20157509. VBIVibVul94426_0332.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG335309.
OMAVKAVGME.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycVVUL216895:VV1_0340-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_VIBVU
AccessionPrimary (citable) accession number: Q8DF81
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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