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Protein

NAD kinase

Gene

nadK

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74Proton acceptorUniRule annotation1
Binding sitei159NADUniRule annotation1
Binding sitei176NADUniRule annotation1
Binding sitei178NADUniRule annotation1
Binding sitei249NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 75NADUniRule annotation2
Nucleotide bindingi148 – 149NADUniRule annotation2
Nucleotide bindingi189 – 194NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:VV1_0366
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001206881 – 294NAD kinaseAdd BLAST294

Interactioni

Protein-protein interaction databases

STRINGi216895.VV1_0366.

Structurei

3D structure databases

ProteinModelPortaliQ8DF58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
HOGENOMiHOG000227221.
KOiK00858.
OMAiKLHWHEY.
OrthoDBiPOG091H02D1.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8DF58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPFNVIAI IGKPRDQQAI QTHRDLYHWL SSLGYQVFID DRLSAILNDV
60 70 80 90 100
PEEHFSGLVE LGEKADLAIV VGGDGNMLGA ARILSRFNTR VIGVNRGNLG
110 120 130 140 150
FLTDLNPEDF QHSLEAVLDG AYIEEERFLL EAEIHRHGQV KSHNAALNEA
160 170 180 190 200
VLHPGQVAHM IEFEVYIDES FAFSLRADGL IVSTPTGSTA YSLSGGGPIL
210 220 230 240 250
SPSLNAISLV PMFPHTLSSR PLVVDGNRRI KLLVSPDNRG TQEVSCDGQV
260 270 280 290
SLPVSPGDEI HIYQSPNRLR LIHPKDYSYY HVLRNKLGWS SKLF
Length:294
Mass (Da):32,555
Last modified:March 1, 2003 - v1
Checksum:i2A43A2025023D5A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO08890.1.
RefSeqiWP_011078461.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaiAAO08890; AAO08890; VV1_0366.
KEGGivvu:VV1_0366.
PATRICi20157552. VBIVibVul94426_0353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO08890.1.
RefSeqiWP_011078461.1. NC_004459.3.

3D structure databases

ProteinModelPortaliQ8DF58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216895.VV1_0366.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO08890; AAO08890; VV1_0366.
KEGGivvu:VV1_0366.
PATRICi20157552. VBIVibVul94426_0353.

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
HOGENOMiHOG000227221.
KOiK00858.
OMAiKLHWHEY.
OrthoDBiPOG091H02D1.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADK_VIBVU
AccessioniPrimary (citable) accession number: Q8DF58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.