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Q8DES7 (SYI_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:VV1_0507
OrganismVibrio vulnificus (strain CMCP6) [Complete proteome] [HAMAP]
Taxonomic identifier216895 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length948 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 948948Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098500

Regions

Motif58 – 6811"HIGH" region HAMAP-Rule MF_02002
Motif607 – 6115"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9111Zinc By similarity
Metal binding9141Zinc By similarity
Metal binding9311Zinc By similarity
Metal binding9341Zinc By similarity
Binding site5661Aminoacyl-adenylate By similarity
Binding site6101ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8DES7 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: A7DAC431BE52744B

FASTA948105,823
        10         20         30         40         50         60 
MSEYKDTLNL PETGFPMRGD LAKREPEMLK RWYQEDLYGE IRKAKKGKKS FVLHDGPPYA 

        70         80         90        100        110        120 
NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YVPGWDCHGL PIELMVEKKV GKPGQKVTAA 

       130        140        150        160        170        180 
EFREKCREYA AGQVEGQKES FKRLGIMGEW DKPYRTMDFT TEANIIRALG QIADNGHLLK 

       190        200        210        220        230        240 
GFKPVHWCTD CGSALAEAEV EYKNKVSPSI DVRFKAADEA ALLAKFSLNE GHQGQGDVSI 

       250        260        270        280        290        300 
VIWTTTPWTL PANRAVCLRD DLEYVLIQVE GDNPERIIVA AELAKDVMDR AGIEHFHNLG 

       310        320        330        340        350        360 
FAKGADLELT QFQHPFYDFT VPAILGDHVT TDSGTGVVHT APGHGQEDFA VGQKYNLEVA 

       370        380        390        400        410        420 
NPVGSNGVYL PDTELFAGQH VFKANDAVVE TLKEKGALLH HHAYEHSYPH CWRHKTPIIF 

       430        440        450        460        470        480 
RATPQWFVSM DQAGLRAKAL ESIKGVQWMP EWGQSRIEGM IEGRPEWCIS RQRTWGVPIA 

       490        500        510        520        530        540 
LFVHKETAEL HPNTSELIEK VAQVVEQKGI QAWWDIDAAE LLGDDAAQYE KVLDTLDVWF 

       550        560        570        580        590        600 
DSGVTHYAVV DKREEFNGAE ADMYLEGSDQ HRGWFQSSLI SSIAMKGKAP YKQVLTHGFV 

       610        620        630        640        650        660 
VDGHGRKMSK SIGNVVAPKD VTNKLGADIL RLWVASTDYT GEVAVSDEIL KRSADAYRRI 

       670        680        690        700        710        720 
RNTARFFLAN LNGFNPETDI VPVEEMVALD RWAVGRALAA QNEIVKAYDE YNTHAVTQRL 

       730        740        750        760        770        780 
MHFCSIEMGS FYLDVIKDRQ YTAKLGGHAQ RSCQTALYYI VEALVRWMAP IMSFTADEIW 

       790        800        810        820        830        840 
NQMPASLPSG ESRDKFVFTG EWFDGLFGLA EGEELNNAFW SEMQKVRGAV NKLLEAARSD 

       850        860        870        880        890        900 
KTIGGSLQAE LTLFADDALA AKINKLEDEL RFVLLTSAAT VKPLSEKSDA AQATDIEGLF 

       910        920        930        940 
VAVRATEAEK CDRCWHHTPD VGTIEGHEKI CGRCVSNVDG EGEARKFA 

« Hide

References

[1]"Complete genome sequence of Vibrio vulnificus CMCP6."
Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CMCP6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016795 Genomic DNA. Translation: AAO09026.1.
RefSeqNP_759499.1. NC_004459.3.

3D structure databases

ProteinModelPortalQ8DES7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216895.VV1_0507.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO09026; AAO09026; VV1_0507.
GeneID1177461.
KEGGvvu:VV1_0507.
PATRIC20157832. VBIVibVul94426_0487.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAKPVHWCL.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycVVUL216895:GIYM-469-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_VIBVU
AccessionPrimary (citable) accession number: Q8DES7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries