Q8DEI1 (CPDA_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA
Short name=3',5'-cyclic AMP phosphodiesterase
Short name=cAMP phosphodiesterase
EC=3.1.4.17

Gene names

Name:	cpdA
Ordered Locus Names:	VV1_0608

Organism

Vibrio vulnificus (strain CMCP6) [Complete proteome] [HAMAP]

Taxonomic identifier

216895 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio vulnificus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes Probable. Ref.2
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. HAMAP-Rule MF_00905
Cofactor	Binds 2 metal cations per subunit By similarity. HAMAP-Rule MF_00905
Induction	Positively regulated by the cAMP-CRP complex. Ref.2
Disruption phenotype	Mutants show increased levels of cellular cAMP. Ref.2
Sequence similarities	Belongs to the cAMP phosphodiesterase class-III family.

Ontologies

Keywords
Ligand	Metal-binding Nucleotide-binding cAMP
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA HAMAP-Rule MF_00905		PRO_0000413383
Regions
	Nucleotide binding	93 – 94	2	cAMP By similarity
Sites
	Metal binding	21	1	Metal cation 1 By similarity
	Metal binding	23	1	Metal cation 1 By similarity
	Metal binding	63	1	Metal cation 1 By similarity
	Metal binding	63	1	Metal cation 2 By similarity
	Metal binding	93	1	Metal cation 2 By similarity
	Metal binding	163	1	Metal cation 2 By similarity
	Metal binding	202	1	Metal cation 2 By similarity
	Metal binding	204	1	Metal cation 1 By similarity
	Binding site	23	1	cAMP By similarity
	Binding site	63	1	cAMP By similarity
	Binding site	204	1	cAMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8DEI1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 742F2E48490227E7	FASTA	274	30,578
	10 20 30 40 50 60 MQHTSSDTLS ENSIKLLQIT DTHLFASDEG SLLSVKTLQS FQAVVEQVMA RHVEFDYLLA 70 80 90 100 110 120 TGDISQDHSA ASYQRFADGI APLEKACFWL PGNHDYKPNM SSVLPSPQIT TPEQVELNAH 130 140 150 160 170 180 WQLILLDSQV VGVPHGRLSD QQLLMLEHHL QASPEKNTLI LLHHHPLLVG SAWLDQHTLK 190 200 210 220 230 240 DAEAFWQIVE RFPMVKGIVC GHVHQDMNVM HKGIRVMATP STCVQFKPKS DDFALDTVSP 250 260 270 GWRELTLHAN GEITTQVQRL ASGSFLPDFT SSGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Vibrio vulnificus CMCP6." Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CMCP6.
[2]	"Expression of the cpdA gene, encoding a 3',5'-cyclic AMP (cAMP) phosphodiesterase, is positively regulated by the cAMP-cAMP receptor protein complex." Kim H.S., Kim S.M., Lee H.J., Park S.J., Lee K.H. J. Bacteriol. 191:922-930(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE. Strain: ATCC 29307 / CDC A8694.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016795 Genomic DNA. Translation: AAO09123.1.
RefSeq	NP_759596.1. NC_004459.3.
3D structure databases
ProteinModelPortal	Q8DEI1.
ModBase	Search...
Protein-protein interaction databases
STRING	216895.VV1_0608.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO09123; AAO09123; VV1_0608.
GeneID	1177558.
KEGG	vvu:VV1_0608.
PATRIC	20158034. VBIVibVul94426_0588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1409.
HOGENOM	HOG000238351.
KO	K03651.
OMA	CAWLDQH.
ProtClustDB	PRK11148.
Enzyme and pathway databases
BioCyc	VVUL216895:GIYM-565-MONOMER.
Family and domain databases
HAMAP	MF_00905. cAMP_phophodiest_CpdA.
InterPro	IPR013622. Calcineurin-like_phos_C. IPR026575. cAMP_Pdiest_CpdA. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProDom	PD587589. Calcineurin-like_phos_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CPDA_VIBVU

Accession

Primary (citable) accession number: Q8DEI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program