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Q8DEI1 (CPDA_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA

Short name=3',5'-cyclic AMP phosphodiesterase
Short name=cAMP phosphodiesterase
EC=3.1.4.17
Gene names
Name:cpdA
Ordered Locus Names:VV1_0608
OrganismVibrio vulnificus (strain CMCP6) [Complete proteome] [HAMAP]
Taxonomic identifier216895 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes Probable. Ref.2

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. HAMAP-Rule MF_00905

Cofactor

Binds 2 metal cations per subunit By similarity. HAMAP-Rule MF_00905

Induction

Positively regulated by the cAMP-CRP complex. Ref.2

Disruption phenotype

Mutants show increased levels of cellular cAMP. Ref.2

Sequence similarities

Belongs to the cAMP phosphodiesterase class-III family.

Ontologies

Keywords
   LigandcAMP
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_function3',5'-cyclic-AMP phosphodiesterase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleotide binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2742743',5'-cyclic adenosine monophosphate phosphodiesterase CpdA HAMAP-Rule MF_00905
PRO_0000413383

Regions

Nucleotide binding93 – 942cAMP By similarity

Sites

Metal binding211Metal cation 1 By similarity
Metal binding231Metal cation 1 By similarity
Metal binding631Metal cation 1 By similarity
Metal binding631Metal cation 2 By similarity
Metal binding931Metal cation 2 By similarity
Metal binding1631Metal cation 2 By similarity
Metal binding2021Metal cation 2 By similarity
Metal binding2041Metal cation 1 By similarity
Binding site231cAMP By similarity
Binding site631cAMP By similarity
Binding site2041cAMP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8DEI1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 742F2E48490227E7

FASTA27430,578
        10         20         30         40         50         60 
MQHTSSDTLS ENSIKLLQIT DTHLFASDEG SLLSVKTLQS FQAVVEQVMA RHVEFDYLLA 

        70         80         90        100        110        120 
TGDISQDHSA ASYQRFADGI APLEKACFWL PGNHDYKPNM SSVLPSPQIT TPEQVELNAH 

       130        140        150        160        170        180 
WQLILLDSQV VGVPHGRLSD QQLLMLEHHL QASPEKNTLI LLHHHPLLVG SAWLDQHTLK 

       190        200        210        220        230        240 
DAEAFWQIVE RFPMVKGIVC GHVHQDMNVM HKGIRVMATP STCVQFKPKS DDFALDTVSP 

       250        260        270 
GWRELTLHAN GEITTQVQRL ASGSFLPDFT SSGY 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Vibrio vulnificus CMCP6."
Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CMCP6.
[2]"Expression of the cpdA gene, encoding a 3',5'-cyclic AMP (cAMP) phosphodiesterase, is positively regulated by the cAMP-cAMP receptor protein complex."
Kim H.S., Kim S.M., Lee H.J., Park S.J., Lee K.H.
J. Bacteriol. 191:922-930(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
Strain: ATCC 29307 / CDC A8694.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016795 Genomic DNA. Translation: AAO09123.1.
RefSeqNP_759596.1. NC_004459.3.

3D structure databases

ProteinModelPortalQ8DEI1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216895.VV1_0608.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO09123; AAO09123; VV1_0608.
GeneID1177558.
KEGGvvu:VV1_0608.
PATRIC20158034. VBIVibVul94426_0588.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1409.
HOGENOMHOG000238351.
KOK03651.
OMAWREIELH.
OrthoDBEOG6QG8GQ.

Enzyme and pathway databases

BioCycVVUL216895:GIYM-565-MONOMER.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
HAMAPMF_00905. cAMP_phophodiest_CpdA.
InterProIPR013622. Calcineurin-like_phos_C.
IPR004843. Calcineurin-like_PHP_apaH.
IPR026575. cAMP_Pdiest_CpdA.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
ProDomPD587589. Calcineurin-like_phos_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF56300. SSF56300. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCPDA_VIBVU
AccessionPrimary (citable) accession number: Q8DEI1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families