ID MDH_VIBVU Reviewed; 310 AA. AC Q8DEC2; DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2003, sequence version 1. DT 27-MAR-2024, entry version 130. DE RecName: Full=Malate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01516}; DE EC=1.1.1.37 {ECO:0000255|HAMAP-Rule:MF_01516}; GN Name=mdh {ECO:0000255|HAMAP-Rule:MF_01516}; GN OrderedLocusNames=VV1_0673; OS Vibrio vulnificus (strain CMCP6). OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae; OC Vibrio. OX NCBI_TaxID=216895; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CMCP6; RA Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.; RT "Complete genome sequence of Vibrio vulnificus CMCP6."; RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the reversible oxidation of malate to oxaloacetate. CC {ECO:0000255|HAMAP-Rule:MF_01516}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate; CC Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589, CC ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37; CC Evidence={ECO:0000255|HAMAP-Rule:MF_01516}; CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01516}. CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family. CC {ECO:0000255|HAMAP-Rule:MF_01516}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE016795; AAO09185.1; -; Genomic_DNA. DR RefSeq; WP_011078751.1; NC_004459.3. DR PDB; 4E0B; X-ray; 2.17 A; A/B/C/D=1-310. DR PDBsum; 4E0B; -. DR AlphaFoldDB; Q8DEC2; -. DR SMR; Q8DEC2; -. DR KEGG; vvu:VV1_0673; -. DR HOGENOM; CLU_047181_1_0_6; -. DR Proteomes; UP000002275; Chromosome 1. DR GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro. DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniRule. DR CDD; cd01337; MDH_glyoxysomal_mitochondrial; 1. DR Gene3D; 3.90.110.10; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR HAMAP; MF_01516; Malate_dehydrog_1; 1. DR InterPro; IPR001557; L-lactate/malate_DH. DR InterPro; IPR022383; Lactate/malate_DH_C. DR InterPro; IPR001236; Lactate/malate_DH_N. DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C. DR InterPro; IPR001252; Malate_DH_AS. DR InterPro; IPR010097; Malate_DH_type1. DR InterPro; IPR023958; Malate_DH_type1_bac. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR NCBIfam; TIGR01772; MDH_euk_gproteo; 1. DR PANTHER; PTHR11540; MALATE AND LACTATE DEHYDROGENASE; 1. DR PANTHER; PTHR11540:SF16; MALATE DEHYDROGENASE, MITOCHONDRIAL; 1. DR Pfam; PF02866; Ldh_1_C; 1. DR Pfam; PF00056; Ldh_1_N; 1. DR PIRSF; PIRSF000102; Lac_mal_DH; 1. DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1. DR PROSITE; PS00068; MDH; 1. PE 1: Evidence at protein level; KW 3D-structure; NAD; Oxidoreductase; Tricarboxylic acid cycle. FT CHAIN 1..310 FT /note="Malate dehydrogenase" FT /id="PRO_0000113333" FT ACT_SITE 177 FT /note="Proton acceptor" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 7..13 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 34 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 81 FT /ligand="substrate" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 87 FT /ligand="substrate" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 94 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 117..119 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 119 FT /ligand="substrate" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 153 FT /ligand="substrate" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT BINDING 227 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01516" FT STRAND 2..6 FT /evidence="ECO:0007829|PDB:4E0B" FT TURN 7..9 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 11..23 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 29..36 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 39..47 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 54..61 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 64..67 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 71..75 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 88..108 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 112..116 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 121..134 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 142..145 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 148..161 FT /evidence="ECO:0007829|PDB:4E0B" FT TURN 166..168 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 173..175 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 179..181 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 182..184 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 186..188 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 196..207 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 209..216 FT /evidence="ECO:0007829|PDB:4E0B" FT TURN 217..219 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 225..242 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 247..254 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 261..270 FT /evidence="ECO:0007829|PDB:4E0B" FT STRAND 273..277 FT /evidence="ECO:0007829|PDB:4E0B" FT HELIX 285..308 FT /evidence="ECO:0007829|PDB:4E0B" SQ SEQUENCE 310 AA; 32168 MW; A9988AA1FF62927A CRC64; MKVAVIGAAG GIGQALALLL KNRLPAGSDL ALYDIAPVTP GVAADLSHIP THVSIKGYAG EDPTPALEGA DVVLISAGVA RKPGMDRADL FNVNAGIVKS LAERIAVVCP NACIGIITNP VNTTVPIAAE VLKKAGVYDK RKLFGVTTLD VIRSETFVAE LKGQDPGEVR VPVIGGHSGV TILPLLSQVE GVEFSDEEIA ALTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGLALVK ALQGEEVIEY AYVEGNGEHA SFFAQPVKLG KEGVEEILPY GELSDFEKAA LDGMLETLNS DIQIGVDFVK //