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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei82NucleophileUniRule annotation1
Active sitei207UniRule annotation1
Active sitei235UniRule annotation1
Binding sitei235SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processBiotin biosynthesis

Enzyme and pathway databases

BioCyciVVUL216895:G1GJ4-842-MONOMER
UniPathwayiUPA00078

Protein family/group databases

ESTHERivibvu-VV10862 BioH

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:VV1_0862
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002044981 – 255Pimeloyl-[acyl-carrier protein] methyl ester esteraseAdd BLAST255

Proteomic databases

PRIDEiQ8DDU4

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8DDU4
SMRiQ8DDU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 242AB hydrolase-1Sequence analysisAdd BLAST227

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingUniRule annotation2
Regioni143 – 147Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3C Bacteria
COG0596 LUCA
HOGENOMiHOG000028062
KOiK02170
OMAiLHGWGMN
OrthoDBiPOG091H04D0

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_01260 Carboxylester, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000073 AB_hydrolase_1
IPR010076 BioH
PfamiView protein in Pfam
PF00561 Abhydrolase_1, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01738 bioH, 1 hit

Sequencei

Sequence statusi: Complete.

Q8DDU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDLHWQTQ GQGPDLVLLH GWGMNGAVWQ QVVERLELHF RLHVVDLPGY
60 70 80 90 100
GHSAHLHAAS LEEIAQQLLE HAPKQAIWVG WSLGGLVATH MALHHADYVS
110 120 130 140 150
KLVTVASSPK FAAEARWRGI QPQVLSAFTE QLSEDFHTTV ERFMALQAMG
160 170 180 190 200
SPSARQDVKN LKQAVFSRPQ PNPQSLLAGL QMLAEVDLRD HLPHLTMPML
210 220 230 240 250
RLYGRLDGLV PIKVAQDLEK VLPASEQFIF TQSSHAPFIT EPESFCHQLL

SFAGK
Length:255
Mass (Da):28,418
Last modified:March 1, 2003 - v1
Checksum:iF2C27A816B257435
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA Translation: AAO09365.1
RefSeqiWP_011078929.1, NC_004459.3

Genome annotation databases

EnsemblBacteriaiAAO09365; AAO09365; VV1_0862
KEGGivvu:VV1_0862

Similar proteinsi

Entry informationi

Entry nameiBIOH_VIBVU
AccessioniPrimary (citable) accession number: Q8DDU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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