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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei119Important for catalytic activityUniRule annotation1
Sitei139Important for catalytic activityUniRule annotation1
Binding sitei296SubstrateUniRule annotation1
Binding sitei325NAD; via amide nitrogenUniRule annotation1
Binding sitei344NADUniRule annotation1
Binding sitei408NADUniRule annotation1
Binding sitei430NADUniRule annotation1
Active sitei451For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei454NADUniRule annotation1
Binding sitei501SubstrateUniRule annotation1
Binding sitei661SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi401 – 403NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciVVUL216895:G1GJ4-950-MONOMER
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:VV1_0981
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001092931 – 723Fatty acid oxidation complex subunit alphaAdd BLAST723

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8DDK6
SMRiQ8DDK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST189
Regioni311 – 7233-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST413

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108DP9 Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261344
KOiK01825
OMAiDYKAKRQ
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01621 FadB, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012799 FadB
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02437 FadB, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q8DDK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYQAETLQV KEVQDGVAEI LFCAPNSVNK LDLATLASLD KALDALTAHS
60 70 80 90 100
GLKGVMLTSD KEAFIVGADI TEFLGLFAKP EEELDQWLQF ANSIFNKLED
110 120 130 140 150
LPVPTVAVVK GHTLGGGCEC VLATDLRIGD KTTSIGLPET KLGIMPGFGG
160 170 180 190 200
CVRLPRVIGA DSAMEIITQG KACRAEEALK IGLLDAVVDS DRLYASALQT
210 220 230 240 250
LTDAINEKID WKARRQQKTS ALTLSKLEAM MSFTMAKGLV AQVAGPHYPA
260 270 280 290 300
PMTAVVTIEE GARFARNQAL DIERKHFVKL AKSEEAKALV GLFLNDQYIK
310 320 330 340 350
GIAKKAAKSA NKETQRAAVL GAGIMGGGIA YQSALKGVPV IMKDIAQASL
360 370 380 390 400
DLGMTEASKL LNKQLERGKI DGFKMAGILA SITPSLHYAG IDNADIIVEA
410 420 430 440 450
VVENPKVKAA VLSEVEEQVS EETVLTSNTS TIPINLLAKS LKRPENFCGM
460 470 480 490 500
HFFNPVHRMP LVEIIRGEHT SDETINRVVA YAAKMGKSPI VVNDCPGFFV
510 520 530 540 550
NRVLFPYFGG FSMLLRDGAD FTQIDKVMER KFGWPMGPAY LLDVVGIDTA
560 570 580 590 600
HHAQAVMAQG FPERMGKQGR DAIDALFEAN KYGQKNGSGF YTYTMDKKGK
610 620 630 640 650
PKKAFSDEIV PILAPVCAAQ QAFDDQTIIQ RMMIPMINEV VLCLQEGIIA
660 670 680 690 700
SAQEADMALV YGLGFPPFRG GVFRYLDSVG IANFVAMAQQ HVELGAMYQV
710 720
PQMLIDMAEK GQTFYGAQQQ GSI
Length:723
Mass (Da):78,302
Last modified:March 1, 2003 - v1
Checksum:i987EEE04C4A68BEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA Translation: AAO09471.1
RefSeqiWP_011079018.1, NC_004459.3

Genome annotation databases

EnsemblBacteriaiAAO09471; AAO09471; VV1_0981
KEGGivvu:VV1_0981

Similar proteinsi

Entry informationi

Entry nameiFADB_VIBVU
AccessioniPrimary (citable) accession number: Q8DDK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health