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Q8DDE5 (RSMB_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase B
EC=2.1.1.176
Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB
Gene names
Name:rsmB
Synonyms:rrmB
Ordered Locus Names:VV1_1046
OrganismVibrio vulnificus (strain CMCP6) [Complete proteome] [HAMAP]
Taxonomic identifier216895 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA By similarity.

Catalytic activity

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
   Biological processRibosome biogenesis
rRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processrRNA methylation

Inferred from electronic annotation. Source: GOC

regulation of transcription, DNA-dependent

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426Ribosomal RNA small subunit methyltransferase B
PRO_0000211808

Regions

Region251 – 2577S-adenosyl-L-methionine binding By similarity

Sites

Active site3721Nucleophile Potential
Binding site2741S-adenosyl-L-methionine By similarity
Binding site3001S-adenosyl-L-methionine By similarity
Binding site3191S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8DDE5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: D53200B63B05CDFC

FASTA42647,677
        10         20         30         40         50         60 
MNVRAAAANV LYQVVDRGNS LSTALPEAQQ SIRPRDHALL QEICYGALRY LPRLESIAGK 

        70         80         90        100        110        120 
LMDKPLKGKQ RVFHHLILVG LYQLGHMRIP AHAAVGETVE GAQALKGPRL RGLINAVLRN 

       130        140        150        160        170        180 
YQREQQALDE FSVSHNAGKY VHPSWLLKIL QDAYPEQWQT IVEANNNKAP MWLRVNHAHH 

       190        200        210        220        230        240 
DRDEYLQLLK NANIDSTLHS EAMDAIKLAA PCDVHSLPGF EQGWVSVQDA AAQLSFNYLQ 

       250        260        270        280        290        300 
PKDGELILDC CAAPGGKTAH ILERVSAREV VALDCDEQRL KRVTENLKRL NLQAKVICGD 

       310        320        330        340        350        360 
ARNPEQWWQG EQFDRILLDA PCSATGVIRR HPDIKWLRRA EDITALAQLQ SEIFDAMWAQ 

       370        380        390        400        410        420 
LKPGGTLVYA TCSITPMENV EQVKAFLART SAAQLVGSER ENPGRQIIPG EEDMDGFYYA 


VLVKQA

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References

[1]"Complete genome sequence of Vibrio vulnificus CMCP6."
Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CMCP6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016795 Genomic DNA. Translation: AAO09533.1.
RefSeqNP_760006.1. NC_004459.3.

3D structure databases

ProteinModelPortalQ8DDE5.
SMRQ8DDE5. Positions 2-424.
ModBaseSearch...

Protein-protein interaction databases

STRING216895.VV1_1046.

Proteomic databases

PRIDEQ8DDE5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO09533; AAO09533; VV1_1046.
GeneID1177979.
KEGGvvu:VV1_1046.
PATRIC20158905. VBIVibVul94426_1003.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0144.
HOGENOMHOG000037300.
KOK03500.
OMAKPPMWLR.
ProtClustDBPRK10901.

Enzyme and pathway databases

BioCycVVUL216895:GIYM-959-MONOMER.

Family and domain databases

Gene3D1.10.940.10. 1 hit.
InterProIPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
[Graphical view]
PfamPF01189. Nol1_Nop2_Fmu. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSPR02008. RCMTFAMILY.
SUPFAMSSF48013. NusB_RsmB_TIM44. 1 hit.
TIGRFAMsTIGR00563. rsmB. 1 hit.
PROSITEPS01153. NOL1_NOP2_SUN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMB_VIBVU
AccessionPrimary (citable) accession number: Q8DDE5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2003
Last modified: May 29, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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