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Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. N5-carboxyaminoimidazole ribonucleotide synthase (purK)
  2. N5-carboxyaminoimidazole ribonucleotide mutase (purE)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93ATPUniRule annotation1
Binding sitei133ATPUniRule annotation1
Binding sitei183ATPUniRule annotation1
Binding sitei206ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi138 – 144ATPUniRule annotation7
Nucleotide bindingi175 – 178ATPUniRule annotation4
Nucleotide bindingi257 – 258ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotation
Ordered Locus Names:VV1_1053
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000750181 – 377N5-carboxyaminoimidazole ribonucleotide synthaseAdd BLAST377

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi216895.VV1_1053.

Structurei

3D structure databases

ProteinModelPortaliQ8DDD8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 287ATP-graspUniRule annotationAdd BLAST191

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CY8. Bacteria.
COG0026. LUCA.
HOGENOMiHOG000034026.
KOiK01589.
OMAiCHIHWYG.
OrthoDBiPOG091H069C.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DDD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVLVLGAGQ LARMMSLAGA PLNIQISAYD VTTGDVVHPL TLHLLGHGLE
60 70 80 90 100
QAIEYVDVIT AEFEHIPHDV LAICQASGKF LPSSEAIKAG GDRRLEKALL
110 120 130 140 150
DHAGVRNANY YVIETREDFN KAIEHVGIPM VLKSALGGYD GKGQWRLKDA
160 170 180 190 200
AQIETLWQEM AACIAATPTQ AIVAEEFVPF QREVSLIGAR GKEGQIEVYP
210 220 230 240 250
LAENIHVNGV LSLSTAIDSP DLQEQAKHMF TAVAETLNYV GVLALEFFDV
260 270 280 290 300
DGQLLVNEIA PRVHNSGHWT QQGAETCQFE NHLRAVCGLP LGSTKLVRET
310 320 330 340 350
SMINILGEDT LPASVMAMDG CHIHWYGKEK RAGRKMGHIN VCGDYSGELQ
360 370
RRLCALANVL DEKAFPAVHE FAKKWQA
Length:377
Mass (Da):41,231
Last modified:March 1, 2003 - v1
Checksum:i6533CC9932BA21C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO09540.1.
RefSeqiWP_011079086.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaiAAO09540; AAO09540; VV1_1053.
KEGGivvu:VV1_1053.
PATRICi20158919. VBIVibVul94426_1010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO09540.1.
RefSeqiWP_011079086.1. NC_004459.3.

3D structure databases

ProteinModelPortaliQ8DDD8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216895.VV1_1053.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO09540; AAO09540; VV1_1053.
KEGGivvu:VV1_1053.
PATRICi20158919. VBIVibVul94426_1010.

Phylogenomic databases

eggNOGiENOG4105CY8. Bacteria.
COG0026. LUCA.
HOGENOMiHOG000034026.
KOiK01589.
OMAiCHIHWYG.
OrthoDBiPOG091H069C.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPURK_VIBVU
AccessioniPrimary (citable) accession number: Q8DDD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.