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Protein

Argininosuccinate synthase

Gene

argG

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Bifunctional protein ArgH (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei91 – 911CitrullineUniRule annotation
Binding sitei96 – 961CitrullineUniRule annotation
Binding sitei121 – 1211ATP; via amide nitrogenUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei127 – 1271AspartateUniRule annotation
Binding sitei127 – 1271CitrullineUniRule annotation
Binding sitei128 – 1281AspartateUniRule annotation
Binding sitei131 – 1311CitrullineUniRule annotation
Binding sitei180 – 1801CitrullineUniRule annotation
Binding sitei189 – 1891CitrullineUniRule annotation
Binding sitei265 – 2651CitrullineUniRule annotation
Binding sitei277 – 2771CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 209ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVVUL216895:GIYM-1228-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:VV1_1373
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Argininosuccinate synthasePRO_0000148663Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi216895.VV1_1373.

Structurei

3D structure databases

ProteinModelPortaliQ8DCN0.
SMRiQ8DCN0. Positions 8-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DCN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLNVNKVV VAYSGGLDTS VIIPWLKENY DCEVVAFVAD VGQGEEELVG
60 70 80 90 100
IEEKAKASGA SECYVVDLKE ELVADYIYPT LKTGAYYEGK YLLGTSMARP
110 120 130 140 150
VIAKAQVEIA RKVGADALCH GCTGKGNDQV RFEGAFAALA PDLKVIAPWR
160 170 180 190 200
EWDLVSREQC LDYLAERNIP CAASLTKIYS RDANAWHIST EGGVLESTWN
210 220 230 240 250
APNEDCWVWT VDPEQAPNEA EYVTLQVEKG EVVAVDGEQM TPYNALVYLN
260 270 280 290 300
EKGAKHGVGR IDIVENRLVG MKSRGCYETP GGTIMMEALR AVEQLVLDKT
310 320 330 340 350
SFEFREELGL KASHLVYDGR WFTPLRKSIM AAADELAQDV NGEVVVKLYK
360 370 380 390 400
GMATVTQKRS ENSLYSEAFA TFGEDEVYDQ SHAGGFIRLY SLSSRIRALN

SQK
Length:403
Mass (Da):44,504
Last modified:March 1, 2004 - v2
Checksum:iDCB3370FB012F31E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO09822.2.
RefSeqiNP_760295.2. NC_004459.3.
WP_011079347.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaiAAO09822; AAO09822; VV1_1373.
GeneIDi2625849.
KEGGivvu:VV1_1373.
PATRICi20159540. VBIVibVul94426_1288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO09822.2.
RefSeqiNP_760295.2. NC_004459.3.
WP_011079347.1. NC_004459.3.

3D structure databases

ProteinModelPortaliQ8DCN0.
SMRiQ8DCN0. Positions 8-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216895.VV1_1373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO09822; AAO09822; VV1_1373.
GeneIDi2625849.
KEGGivvu:VV1_1373.
PATRICi20159540. VBIVibVul94426_1288.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciVVUL216895:GIYM-1228-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Vibrio vulnificus CMCP6."
    Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CMCP6.

Entry informationi

Entry nameiASSY_VIBVU
AccessioniPrimary (citable) accession number: Q8DCN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: March 1, 2004
Last modified: June 24, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.