Reviewed, UniProtKB/Swiss-Prot Q8DCK1 (CYSI_VIBVU)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	VV1_1403

Organism

Vibrio vulnificus [Complete proteome] [HAMAP]

Taxonomic identifier

672 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta4. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000199915
Sites
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	447	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	487	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	491	1	Iron (siroheme axial ligand) By similarity
	Metal binding	491	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8DCK1-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 146CF39788F73AD2	FASTA	578	64,943
	10 20 30 40 50 60 MTFSIENNKQ VVLGEELGKL SDNERLKTQS NFLRGTIEQD LQDRITGGFT ADNFQLIRFH 70 80 90 100 110 120 GMYQQDDRDI RNERAKQKLE PLHNVMLRAR MPGGIITPKQ WLAIDKFATE HSLYGSIRLT 130 140 150 160 170 180 TRQTFQFHGV LKPNIKLMHQ TLNSIGIDSI ATAGDVNRNV LCTTNPVESE LHQEAYEWAK 190 200 210 220 230 240 KISEHLLPKT KAYAEIWLDG EKVETTEDDE PILGKTYLPR KFKTTVVIPP QNDVDVHAND 250 260 270 280 290 300 LNFVAIAEKG KLIGFNVLVG GGLAMTHGDT STYPRRADDF GYIPLEKTLD VAAAVVTTQR 310 320 330 340 350 360 DWGNRSNRKN AKTKYTLDRV GTDVFKAEVE KRAGIKFEVS RPYEFTERGD RIGWVEGIDG 370 380 390 400 410 420 KHHLTLFIEN GRLLDYPGKP LKTGVAEIAK IHKGDFRMTA NQNLIVAGVS KSNKAKIEKL 430 440 450 460 470 480 AREHGLMDEG VSEQRKNAMA CVAFPTCPLA MAEAERFLPQ FVTDVEGILD KHGLDKEDNI 490 500 510 520 530 540 ILRVTGCPNG CGRAMLAEIG LVGKAPGRYN LHLGGNRGGT RVPKMYKENI TDKQILEEID 550 560 570 QLVGRWANER LPNECFGDFT VRVGIIEEVI ISKRDFYA

References

[1]	"Complete genome sequence of Vibrio vulnificus CMCP6." Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CMCP6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016795 Genomic DNA. Translation: AAO09852.1.
RefSeq	NP_760325.1.
3D structure databases
HSSP	HSSP built from PDB template 1AOP based on UniProtKB P17846.
ModBase	Search...
Genome annotation databases
GeneID	1178324.
GenomeReviews	Gene locus VV1_1403 in contig AE016795_GR.
KEGG	vvu:VV1_1403.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8DCK1.
OMA	Q8DCK1. AFQFHGI.
Enzyme and pathway databases
BioCyc	VVUL216895:VV1_1403-MON.
BRENDA	1.8.1.2. 277169.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_VIBVU

Accession

Primary (citable) accession number: Q8DCK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)