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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Bifunctional protein ArgH (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11Carbamoyl phosphateBy similarity1
Binding sitei73Carbamoyl phosphateBy similarity1
Binding sitei84Carbamoyl phosphateBy similarity1
Binding sitei108Carbamoyl phosphateBy similarity1
Binding sitei169OrnithineBy similarity1
Binding sitei233OrnithineBy similarity1
Binding sitei302Carbamoyl phosphateBy similarity1
Binding sitei320Carbamoyl phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:VV1_1466
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001130561 – 334Ornithine carbamoyltransferaseAdd BLAST334

Proteomic databases

PRIDEiQ8DCF5.

Interactioni

Protein-protein interaction databases

STRINGi216895.VV1_1466.

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 5Combined sources5
Helixi13 – 15Combined sources3
Helixi18 – 37Combined sources20
Turni43 – 46Combined sources4
Beta strandi48 – 55Combined sources8
Helixi59 – 70Combined sources12
Beta strandi74 – 78Combined sources5
Beta strandi80 – 83Combined sources4
Turni86 – 88Combined sources3
Helixi91 – 101Combined sources11
Beta strandi103 – 108Combined sources6
Helixi112 – 121Combined sources10
Beta strandi122 – 124Combined sources3
Beta strandi126 – 129Combined sources4
Helixi136 – 148Combined sources13
Turni149 – 152Combined sources4
Helixi155 – 157Combined sources3
Beta strandi159 – 164Combined sources6
Helixi169 – 181Combined sources13
Beta strandi184 – 189Combined sources6
Helixi191 – 193Combined sources3
Helixi197 – 210Combined sources14
Beta strandi213 – 218Combined sources6
Helixi220 – 224Combined sources5
Beta strandi228 – 232Combined sources5
Beta strandi238 – 240Combined sources3
Helixi244 – 252Combined sources9
Helixi253 – 255Combined sources3
Helixi259 – 264Combined sources6
Beta strandi271 – 274Combined sources4
Beta strandi281 – 284Combined sources4
Helixi285 – 293Combined sources9
Beta strandi299 – 301Combined sources3
Helixi303 – 306Combined sources4
Helixi313 – 332Combined sources20

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Important for structural integrityBy similarity1
Sitei148Important for structural integrityBy similarity1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UPDX-ray2.91A1-334[»]
4H31X-ray1.70A/B/C1-334[»]
4JFRX-ray2.17A/B/C1-334[»]
4JHXX-ray1.85A/B/C1-334[»]
4JQOX-ray2.08A/B/C1-334[»]
4KWTX-ray1.86A/B/C1-334[»]
ProteinModelPortaliQ8DCF5.
SMRiQ8DCF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 61Carbamoyl phosphate bindingBy similarity5
Regioni135 – 138Carbamoyl phosphate bindingBy similarity4
Regioni237 – 238Ornithine bindingBy similarity2
Regioni274 – 277Carbamoyl phosphate bindingBy similarity4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiMGMEIRL.
OrthoDBiPOG091H0543.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DCF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFNLRNRNF LKLLDFSTKE IQFLIDLSAD LKKAKYAGTE QKKLLGKNIA
60 70 80 90 100
LIFEKASTRT RCAFEVAAFD QGAQVTYIGP SGSQIGDKES MKDTARVLGR
110 120 130 140 150
MYDGIQYRGF GQAIVEELGA FAGVPVWNGL TDEFHPTQIL ADFLTMLEHS
160 170 180 190 200
QGKALADIQF AYLGDARNNV GNSLMVGAAK MGMDIRLVGP QAYWPDEELV
210 220 230 240 250
AACQAIAKQT GGKITLTENV AEGVQGCDFL YTDVWVSMGE SPEAWDERVA
260 270 280 290 300
LMKPYQVNMN VLKQTGNPNV KFMHCLPAFH NDETTIGKQV ADKFGMKGLE
310 320 330
VTEEVFESEH SIVFDEAENR MHTIKAVMVA TLGS
Length:334
Mass (Da):36,859
Last modified:March 1, 2003 - v1
Checksum:i67E1FFDE5B43A064
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO09905.1.
RefSeqiWP_011079423.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaiAAO09905; AAO09905; VV1_1466.
KEGGivvu:VV1_1466.
PATRICi20159709. VBIVibVul94426_1366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO09905.1.
RefSeqiWP_011079423.1. NC_004459.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UPDX-ray2.91A1-334[»]
4H31X-ray1.70A/B/C1-334[»]
4JFRX-ray2.17A/B/C1-334[»]
4JHXX-ray1.85A/B/C1-334[»]
4JQOX-ray2.08A/B/C1-334[»]
4KWTX-ray1.86A/B/C1-334[»]
ProteinModelPortaliQ8DCF5.
SMRiQ8DCF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216895.VV1_1466.

Proteomic databases

PRIDEiQ8DCF5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO09905; AAO09905; VV1_1466.
KEGGivvu:VV1_1466.
PATRICi20159709. VBIVibVul94426_1366.

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiMGMEIRL.
OrthoDBiPOG091H0543.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_VIBVU
AccessioniPrimary (citable) accession number: Q8DCF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.