Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Bifunctional protein ArgH (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111Carbamoyl phosphateBy similarity
Binding sitei73 – 731Carbamoyl phosphateBy similarity
Binding sitei84 – 841Carbamoyl phosphateBy similarity
Binding sitei108 – 1081Carbamoyl phosphateBy similarity
Binding sitei169 – 1691OrnithineBy similarity
Binding sitei233 – 2331OrnithineBy similarity
Binding sitei302 – 3021Carbamoyl phosphateBy similarity
Binding sitei320 – 3201Carbamoyl phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciVVUL216895:GIYM-1305-MONOMER.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:VV1_1466
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Ornithine carbamoyltransferasePRO_0000113056Add
BLAST

Proteomic databases

PRIDEiQ8DCF5.

Interactioni

Protein-protein interaction databases

STRINGi216895.VV1_1466.

Structurei

Secondary structure

334
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 55Combined sources
Helixi13 – 153Combined sources
Helixi18 – 3720Combined sources
Turni43 – 464Combined sources
Beta strandi48 – 558Combined sources
Helixi59 – 7012Combined sources
Beta strandi74 – 785Combined sources
Beta strandi80 – 834Combined sources
Turni86 – 883Combined sources
Helixi91 – 10111Combined sources
Beta strandi103 – 1086Combined sources
Helixi112 – 12110Combined sources
Beta strandi122 – 1243Combined sources
Beta strandi126 – 1294Combined sources
Helixi136 – 14813Combined sources
Turni149 – 1524Combined sources
Helixi155 – 1573Combined sources
Beta strandi159 – 1646Combined sources
Helixi169 – 18113Combined sources
Beta strandi184 – 1896Combined sources
Helixi191 – 1933Combined sources
Helixi197 – 21014Combined sources
Beta strandi213 – 2186Combined sources
Helixi220 – 2245Combined sources
Beta strandi228 – 2325Combined sources
Beta strandi238 – 2403Combined sources
Helixi244 – 2529Combined sources
Helixi253 – 2553Combined sources
Helixi259 – 2646Combined sources
Beta strandi271 – 2744Combined sources
Beta strandi281 – 2844Combined sources
Helixi285 – 2939Combined sources
Beta strandi299 – 3013Combined sources
Helixi303 – 3064Combined sources
Helixi313 – 33220Combined sources

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei32 – 321Important for structural integrityBy similarity
Sitei148 – 1481Important for structural integrityBy similarity

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UPDX-ray2.91A1-334[»]
4H31X-ray1.70A/B/C1-334[»]
4JFRX-ray2.17A/B/C1-334[»]
4JHXX-ray1.85A/B/C1-334[»]
4JQOX-ray2.08A/B/C1-334[»]
4KWTX-ray1.86A/B/C1-334[»]
ProteinModelPortaliQ8DCF5.
SMRiQ8DCF5. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 615Carbamoyl phosphate bindingBy similarity
Regioni135 – 1384Carbamoyl phosphate bindingBy similarity
Regioni237 – 2382Ornithine bindingBy similarity
Regioni274 – 2774Carbamoyl phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiMGMEIRL.
OrthoDBiPOG091H0543.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DCF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFNLRNRNF LKLLDFSTKE IQFLIDLSAD LKKAKYAGTE QKKLLGKNIA
60 70 80 90 100
LIFEKASTRT RCAFEVAAFD QGAQVTYIGP SGSQIGDKES MKDTARVLGR
110 120 130 140 150
MYDGIQYRGF GQAIVEELGA FAGVPVWNGL TDEFHPTQIL ADFLTMLEHS
160 170 180 190 200
QGKALADIQF AYLGDARNNV GNSLMVGAAK MGMDIRLVGP QAYWPDEELV
210 220 230 240 250
AACQAIAKQT GGKITLTENV AEGVQGCDFL YTDVWVSMGE SPEAWDERVA
260 270 280 290 300
LMKPYQVNMN VLKQTGNPNV KFMHCLPAFH NDETTIGKQV ADKFGMKGLE
310 320 330
VTEEVFESEH SIVFDEAENR MHTIKAVMVA TLGS
Length:334
Mass (Da):36,859
Last modified:March 1, 2003 - v1
Checksum:i67E1FFDE5B43A064
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO09905.1.
RefSeqiWP_011079423.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaiAAO09905; AAO09905; VV1_1466.
KEGGivvu:VV1_1466.
PATRICi20159709. VBIVibVul94426_1366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO09905.1.
RefSeqiWP_011079423.1. NC_004459.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UPDX-ray2.91A1-334[»]
4H31X-ray1.70A/B/C1-334[»]
4JFRX-ray2.17A/B/C1-334[»]
4JHXX-ray1.85A/B/C1-334[»]
4JQOX-ray2.08A/B/C1-334[»]
4KWTX-ray1.86A/B/C1-334[»]
ProteinModelPortaliQ8DCF5.
SMRiQ8DCF5. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216895.VV1_1466.

Proteomic databases

PRIDEiQ8DCF5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO09905; AAO09905; VV1_1466.
KEGGivvu:VV1_1466.
PATRICi20159709. VBIVibVul94426_1366.

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiMGMEIRL.
OrthoDBiPOG091H0543.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
BioCyciVVUL216895:GIYM-1305-MONOMER.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_VIBVU
AccessioniPrimary (citable) accession number: Q8DCF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.