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Q8DC35 (Q8DC35_VIBVU) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Signal recognition particle protein HAMAP-Rule MF_00306
Alternative name(s):
Fifty-four homolog HAMAP-Rule MF_00306
Gene names
Name:ffh HAMAP-Rule MF_00306 EMBL AAO10032.2
Ordered Locus Names:VV1_1612 EMBL AAO10032.2
OrganismVibrio vulnificus (strain CMCP6) [Complete proteome] [HAMAP] EMBL AAO10032.2
Taxonomic identifier216895 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components By similarity. HAMAP-Rule MF_00306

Subunit structure

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule By similarity. HAMAP-Rule MF_00306

Subcellular location

Cytoplasm. Note: The SRP-RNC complex is targeted to the cytoplasmic membrane By similarity. HAMAP-Rule MF_00306

Domain

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC By similarity. HAMAP-Rule MF_00306

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily. HAMAP-Rule MF_00306

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding107 – 1148GTP By similarity HAMAP-Rule MF_00306
Nucleotide binding190 – 1945GTP By similarity HAMAP-Rule MF_00306
Nucleotide binding248 – 2514GTP By similarity HAMAP-Rule MF_00306

Sequences

Sequence LengthMass (Da)Tools
Q8DC35 [UniParc].

Last modified April 5, 2011. Version 2.
Checksum: E947B95BAC917607

FASTA45849,949
        10         20         30         40         50         60 
MFENLTDRLS KTLKNISGKG RLTEDNIKDT LREVRMALLE ADVALPVVRE FVARVKEKAV 

        70         80         90        100        110        120 
GVEVSKSLTP GQEFIKIVQG ELEAVMGESN EALNLAAQPP AVILMAGLQG AGKTTSVGKL 

       130        140        150        160        170        180 
SKLLKERDKK KVLVVSADVY RPAAIKQLET LASDIGVDFF PSSADQKPID IANAAIDHAK 

       190        200        210        220        230        240 
KKFFDVLIVD TAGRLAIDEA MMGEIQQLHS AINPVETLFV VDAMTGQDAA NTAKAFGDAL 

       250        260        270        280        290        300 
PLTGVILTKV DGDARGGAAL SVRHITGKPI KFLGVGEKTD ALEPFHPDRV ASRILGMGDV 

       310        320        330        340        350        360 
LSLIEDLQRN VDTEKAQKLA QKFKEKKGFD LEDFREQLGQ MQNMGGMMGM LDKLPGMSQL 

       370        380        390        400        410        420 
PSDIKDKVDD KMFKQMEAII NSMTMKERQN PDLIKGSRKK RIAAGSGTQV QDVNRLLKQF 

       430        440        450 
TQMQKMMKKM QKGGIKGMMR NMQGMMGGMG GGFNPFGR

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References

[1]"Complete genome sequence of Vibrio vulnificus CMCP6."
Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CMCP6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016795 Genomic DNA. Translation: AAO10032.2.
RefSeqNP_760505.2. NC_004459.3.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING216895.VV1_1612.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO10032; AAO10032; VV1_1612.
GeneID1178529.
KEGGvvu:VV1_1612.
PATRIC20160011. VBIVibVul94426_1491.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0541.
HOGENOMHOG000036277.
KOK03106.

Enzyme and pathway databases

BioCycVVUL216895:GIYM-1422-MONOMER.

Family and domain databases

Gene3D1.10.260.30. 1 hit.
HAMAPMF_00306. SRP54.
InterProIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMSSF47446. Signal_recog_particle_SRP54_M. 1 hit.
SSF47364. SRP54. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00959. ffh. 1 hit.
PROSITEPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ8DC35_VIBVU
AccessionPrimary (citable) accession number: Q8DC35
Entry history
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: April 5, 2011
Last modified: May 29, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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